Dolastatin-10 induces polyploidy, telomeric associations and apoptosis in a murine melanoma cell line.

  • Authors:
    • S Pathak
    • A S Multani
    • M Ozen
    • M A Richardson
    • R A Newman
  • View Affiliations

  • Published online on: March 1, 1998     https://doi.org/10.3892/or.5.2.373
  • Pages: 373-379
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Abstract

Our purpose was to study the effects of dolastatin-10 (Dol-10) on chromosome morphology, telomeric associations, induction of polyploidy and cell death in a metastatic murine melanoma cell line, K1735 clone X-21. Murine melanoma cells were treated with various concentrations (10 ng/ml, 100 ng/ml and 1000 ng/ml) of Dol-10 for 4, 24 and 72 h continuously and harvested immediately without recovery. In another set of experiments, cells were treated for 4 h with the same concentrations, washed with prewarmed medium and then allowed to recover in drug-free medium for 24 h and subsequently harvested. Our preliminary results indicated: i) a drug-mediated increase in the frequency of metaphases, with telomeric associations resulting in multicentric and ring configurations; ii) induction of clumping in metaphase chromosomes; iii) induction of polyploidy as a result of endoreduplication; iv) formation of micronucleated cells; and v) induction of cell death. These observations indicated that Dol-10 could be a potent antineoplastic drug against malignant melanoma. In addition to its reported interaction with cell microtubules, the mechanism of action of Dol-10 may be mediated through the loss of telomeric repeats and induction of chromosome aberrations.

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Mar-Apr 1998
Volume 5 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Pathak S, Multani A, Ozen M, Richardson M and Newman R: Dolastatin-10 induces polyploidy, telomeric associations and apoptosis in a murine melanoma cell line.. Oncol Rep 5: 373-379, 1998.
APA
Pathak, S., Multani, A., Ozen, M., Richardson, M., & Newman, R. (1998). Dolastatin-10 induces polyploidy, telomeric associations and apoptosis in a murine melanoma cell line.. Oncology Reports, 5, 373-379. https://doi.org/10.3892/or.5.2.373
MLA
Pathak, S., Multani, A., Ozen, M., Richardson, M., Newman, R."Dolastatin-10 induces polyploidy, telomeric associations and apoptosis in a murine melanoma cell line.". Oncology Reports 5.2 (1998): 373-379.
Chicago
Pathak, S., Multani, A., Ozen, M., Richardson, M., Newman, R."Dolastatin-10 induces polyploidy, telomeric associations and apoptosis in a murine melanoma cell line.". Oncology Reports 5, no. 2 (1998): 373-379. https://doi.org/10.3892/or.5.2.373