Activity of glycogen synthase kinase-3beta is down-regulated during transient differentiation of human colon cancer HT-29 cells.

  • Authors:
    • Z Tuhackova
    • E Sloncova
    • J Hlavacek
    • V Sovova
    • J Velek
  • View Affiliations

  • Published online on: July 1, 1999     https://doi.org/10.3892/or.6.4.827
  • Pages: 827-859
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Abstract

The increased phosphorylation and activity of protein kinase B (PKB/Akt) was found early upon butyrate treatment of HT-29 cells with a potent differentiating agent, sodium butyrate. It was accompanied by the increased phosphorylation of glycogen synthase kinase-3 (GSK-3) and the inhibition of the activity of GSK-3beta to catalyze phosphorylation of its substrate, translation initiation factor eIF2B. Phosphorylation of PKB and GSK-3 in HT-29 cells was reduced by wortmannin, the inhibitor of phosphatidylinositol-3' kinase (PI3'-kinase), which is upstream activator of PKB and GSK-3 in the intracellular signalling. Modulation of the activity and phosphorylation of these protein kinases during transient in vitro differentiation of HT-29 cells indicates that control of the PI3'-kinase/PKB-dependent signalling pathway may be implicated very early in the changes of malignant phenotype of HT-29 cells.

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Jul-Aug 1999
Volume 6 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Tuhackova Z, Sloncova E, Hlavacek J, Sovova V and Velek J: Activity of glycogen synthase kinase-3beta is down-regulated during transient differentiation of human colon cancer HT-29 cells.. Oncol Rep 6: 827-859, 1999.
APA
Tuhackova, Z., Sloncova, E., Hlavacek, J., Sovova, V., & Velek, J. (1999). Activity of glycogen synthase kinase-3beta is down-regulated during transient differentiation of human colon cancer HT-29 cells.. Oncology Reports, 6, 827-859. https://doi.org/10.3892/or.6.4.827
MLA
Tuhackova, Z., Sloncova, E., Hlavacek, J., Sovova, V., Velek, J."Activity of glycogen synthase kinase-3beta is down-regulated during transient differentiation of human colon cancer HT-29 cells.". Oncology Reports 6.4 (1999): 827-859.
Chicago
Tuhackova, Z., Sloncova, E., Hlavacek, J., Sovova, V., Velek, J."Activity of glycogen synthase kinase-3beta is down-regulated during transient differentiation of human colon cancer HT-29 cells.". Oncology Reports 6, no. 4 (1999): 827-859. https://doi.org/10.3892/or.6.4.827