Enhancement of gene transfer efficiency into human cancer cells by modification of retroviral vectors and addition of chemicals.

  • Authors:
    • J J Song
    • J H Kim
    • H Lee
    • E Kim
    • J Kim
    • Y S Park
    • J Ahn
    • N C Yoo
    • J K Roh
    • B S Kim
  • View Affiliations

  • Published online on: January 1, 2000     https://doi.org/10.3892/or.7.1.119
  • Pages: 119-143
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Abstract

Retroviral vectors have recently experienced limited use in cancer gene therapy mainly due to poor transduction efficiency. To overcome this drawback, we attempted to enhance the transduction efficiency by employing different retroviral packaging cell lines and chemical additives. The retrovirus from the PG13 packaging cell line gave mostly higher or similar transduction efficiencies in a variety of human cancer cell lines compared to the retrovirus from the PA317, Bing, or FLYRD18 packaging cell line. A cationic liposome, especially Lipofectamine, significantly enhanced the transduction efficiency of a retrovirus. However, the retrovirus derived from the PG13 cell line could not infect the murine cell line efficiently even after Lipofectamine treatment. Furthermore, chloroquine did not improve the transduction efficiency regardless of the presence of chemical additives. These results, therefore, suggested that the transduction efficiency of a retrovirus in human cancer cells can certainly be improved when a proper packaging cell line is chosen. In addition, this study implied that Lipofectamine is a superb additive to enhance the transduction efficiency of a retrovirus via a specific virus envelope protein-receptor interaction for virus entry, and that receptor-mediated endocytosis does not seem to be the leading route of virus delivery to liberate a virus genome.

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Jan-Feb 2000
Volume 7 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Song J, Kim J, Lee H, Kim E, Kim J, Park Y, Ahn J, Yoo N, Roh J, Kim B, Kim B, et al: Enhancement of gene transfer efficiency into human cancer cells by modification of retroviral vectors and addition of chemicals.. Oncol Rep 7: 119-143, 2000
APA
Song, J., Kim, J., Lee, H., Kim, E., Kim, J., Park, Y. ... Kim, B. (2000). Enhancement of gene transfer efficiency into human cancer cells by modification of retroviral vectors and addition of chemicals.. Oncology Reports, 7, 119-143. https://doi.org/10.3892/or.7.1.119
MLA
Song, J., Kim, J., Lee, H., Kim, E., Kim, J., Park, Y., Ahn, J., Yoo, N., Roh, J., Kim, B."Enhancement of gene transfer efficiency into human cancer cells by modification of retroviral vectors and addition of chemicals.". Oncology Reports 7.1 (2000): 119-143.
Chicago
Song, J., Kim, J., Lee, H., Kim, E., Kim, J., Park, Y., Ahn, J., Yoo, N., Roh, J., Kim, B."Enhancement of gene transfer efficiency into human cancer cells by modification of retroviral vectors and addition of chemicals.". Oncology Reports 7, no. 1 (2000): 119-143. https://doi.org/10.3892/or.7.1.119