Selection of mRNA markers for detection of lymph node micrometastases in breast cancer patients.

  • Authors:
    • M Ooka
    • I Sakita
    • Y Fujiwara
    • Y Tamaki
    • H Yamamoto
    • T Aihara
    • M Miyazaki
    • M Kadota
    • N Masuda
    • Y Sugita
    • K Iwao
    • M Monden
  • View Affiliations

  • Published online on: May 1, 2000     https://doi.org/10.3892/or.7.3.561
  • Pages: 561-567
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Abstract

The aim of this study was to search for specific and sensitive mRNA markers or a combination of markers for RT-PCR detection of micrometastases in axillary lymph nodes (LNs) from patients with breast cancer. LNs (n=177) from 17 patients were examined with Cytokeratin20 (CK20), melanoma-associated genes (MAGE1, MAGE3), carcinoembryonic antigen (CEA), prostate-specific antigen (PSA), mammaglobin (MGB1) and mammaglobin B (MGB2) as molecular markers. CK20, MAGE1 and MAGE3 were slightly positive in primary tumors and CEA, PSA, MGB1 and MGB2 were highly positive. MGB1 and MGB2 were 100% positive in HE-positive LNs while CEA and PSA were only 35.7% and 57.1% positive. MGB1 and MGB2 were also 30.1% and 17.8% positive in HE-negative nodes. Thus, MGB1 and MGB2 are specific and a combination of the two should be useful for detection of micrometastases in axillary LNs of breast cancer patients.

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May-Jun 2000
Volume 7 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ooka M, Sakita I, Fujiwara Y, Tamaki Y, Yamamoto H, Aihara T, Miyazaki M, Kadota M, Masuda N, Sugita Y, Sugita Y, et al: Selection of mRNA markers for detection of lymph node micrometastases in breast cancer patients.. Oncol Rep 7: 561-567, 2000
APA
Ooka, M., Sakita, I., Fujiwara, Y., Tamaki, Y., Yamamoto, H., Aihara, T. ... Monden, M. (2000). Selection of mRNA markers for detection of lymph node micrometastases in breast cancer patients.. Oncology Reports, 7, 561-567. https://doi.org/10.3892/or.7.3.561
MLA
Ooka, M., Sakita, I., Fujiwara, Y., Tamaki, Y., Yamamoto, H., Aihara, T., Miyazaki, M., Kadota, M., Masuda, N., Sugita, Y., Iwao, K., Monden, M."Selection of mRNA markers for detection of lymph node micrometastases in breast cancer patients.". Oncology Reports 7.3 (2000): 561-567.
Chicago
Ooka, M., Sakita, I., Fujiwara, Y., Tamaki, Y., Yamamoto, H., Aihara, T., Miyazaki, M., Kadota, M., Masuda, N., Sugita, Y., Iwao, K., Monden, M."Selection of mRNA markers for detection of lymph node micrometastases in breast cancer patients.". Oncology Reports 7, no. 3 (2000): 561-567. https://doi.org/10.3892/or.7.3.561