Selective cytotoxicity of adriamycin immunoconjugate of monoclonal antibody MSN-1 to endometrial adenocarcinoma in vitro and in vivo.

  • Authors:
    • Y Kaneta
    • K Tsukazaki
    • K Kubushiro
    • M Sakayori
    • M Ueda
    • S Nozawa
  • View Affiliations

  • Published online on: September 1, 2000     https://doi.org/10.3892/or.7.5.1099
  • Pages: 1099-1205
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Abstract

Missile therapy, which destroys cancer cells specifically, has been regarded as an effective treatment modality for carcinoma. The monoclonal antibody MSN-1 (IgM), which reacts strongly with endometrial adenocarcinomas, was combined with adriamycin (ADM) by a disulfide bond using N-succinimidyl-3-(2-pyridyldithio) propionate (SPDP) and 2-iminothiolane. Its selective cytotoxicity against SNG-II was examined in a colony formation in vitro, and on athymic mice in vivo. The results of our study suggest that the or IC50, of the MSN-1-ADM immunoconjugate against SNG-II to be 57 times that of ADM alone in vitro. The reductions in resected weights of target tumor cells, at the local site of the MSN-1-ADM immunoconjugate treatment, were 25% with caudal vein administration, and 38% with local administration, as compared with the untreated group, in vivo. There was no weight loss in treated mice. Our results suggest that this MSN-1-ADM immunoconjugate has potential clinical application in the treatment of endometrial adenocarcinomas.

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Sep-Oct 2000
Volume 7 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kaneta Y, Tsukazaki K, Kubushiro K, Sakayori M, Ueda M and Nozawa S: Selective cytotoxicity of adriamycin immunoconjugate of monoclonal antibody MSN-1 to endometrial adenocarcinoma in vitro and in vivo.. Oncol Rep 7: 1099-1205, 2000.
APA
Kaneta, Y., Tsukazaki, K., Kubushiro, K., Sakayori, M., Ueda, M., & Nozawa, S. (2000). Selective cytotoxicity of adriamycin immunoconjugate of monoclonal antibody MSN-1 to endometrial adenocarcinoma in vitro and in vivo.. Oncology Reports, 7, 1099-1205. https://doi.org/10.3892/or.7.5.1099
MLA
Kaneta, Y., Tsukazaki, K., Kubushiro, K., Sakayori, M., Ueda, M., Nozawa, S."Selective cytotoxicity of adriamycin immunoconjugate of monoclonal antibody MSN-1 to endometrial adenocarcinoma in vitro and in vivo.". Oncology Reports 7.5 (2000): 1099-1205.
Chicago
Kaneta, Y., Tsukazaki, K., Kubushiro, K., Sakayori, M., Ueda, M., Nozawa, S."Selective cytotoxicity of adriamycin immunoconjugate of monoclonal antibody MSN-1 to endometrial adenocarcinoma in vitro and in vivo.". Oncology Reports 7, no. 5 (2000): 1099-1205. https://doi.org/10.3892/or.7.5.1099