BrdUrd-induced radiosensitization of two human tumour cell lines at iso-levels of incorporation.

  • Authors:
    • N A Franken
    • C Van Bree
    • J B Kipp
    • H M Rodermond
    • J Haveman
    • G W Barendsen
  • View Affiliations

  • Published online on: September 1, 2000     https://doi.org/10.3892/or.7.5.937
  • Pages: 937-977
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Abstract

Bromodeoxyuridine (BrdUrd)-induced radiosensitization of two different tumour cell lines was compared at equal levels of thymidine replacement. Human lung carcinoma cells (SW-1573) and human colorectal carcinoma cells (RKO) were grown for 48 h in the presence of respectively 1 microM BrdUrd and 4 microM of BrdUrd in order to obtain equal levels of BrdUrd into the DNA. In SW cells the level of thymidine replacement by BrdUrd was 6.7+/-0.5% and in RKO cells this was 7.1+/-0.8. Cell survival after irradiation with single doses up to 8 Gy, was determined with clonogenic assay. The magnitude of BrdUrd-induced radiosensitization was determined by analyzing radiation-dose survival curves with the linear-quadratic formula [S(D)/S(0)=exp-(alphaD+betaD2)]. In the SW cells BrdUrd radiosensitization led to a significant increase of the linear parameter, alpha, determining the initial slope of the survival curves, by a factor of about 2. In the RKO cells BrdUrd increased the value of alpha by a factor 1.4. This suggests that repair of potentially lethal damage (PLD) is inhibited. In both cell lines the quadratic term, beta, strongly influencing the high dose region of the survival curves, was not altered by sensitization by BrdUrd. The increase of alpha is of interest for clinical applications as BrdUrd sensitizes tumour cells after low doses of radiation.

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Sep-Oct 2000
Volume 7 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Franken N, Van Bree C, Kipp J, Rodermond H, Haveman J and Barendsen G: BrdUrd-induced radiosensitization of two human tumour cell lines at iso-levels of incorporation.. Oncol Rep 7: 937-977, 2000
APA
Franken, N., Van Bree, C., Kipp, J., Rodermond, H., Haveman, J., & Barendsen, G. (2000). BrdUrd-induced radiosensitization of two human tumour cell lines at iso-levels of incorporation.. Oncology Reports, 7, 937-977. https://doi.org/10.3892/or.7.5.937
MLA
Franken, N., Van Bree, C., Kipp, J., Rodermond, H., Haveman, J., Barendsen, G."BrdUrd-induced radiosensitization of two human tumour cell lines at iso-levels of incorporation.". Oncology Reports 7.5 (2000): 937-977.
Chicago
Franken, N., Van Bree, C., Kipp, J., Rodermond, H., Haveman, J., Barendsen, G."BrdUrd-induced radiosensitization of two human tumour cell lines at iso-levels of incorporation.". Oncology Reports 7, no. 5 (2000): 937-977. https://doi.org/10.3892/or.7.5.937