Transformation-associated changes in gene expression of alternative splicing regulatory factors in mouse fibroblast cells

  • Authors:
    • Takashi Maeda
    • Souhei Furukawa
  • View Affiliations

  • Published online on: May 1, 2001     https://doi.org/10.3892/or.8.3.563
  • Pages: 563-566
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Abstract

Although the alternative splicing of various genes is a common phenomenon in tumorigenesis, little is known about the mechanism behind it. Recently, we found altered expression of splicing regulatory factors during two-step chemical transformation in vitro. However, it remains unknown whether such altered expression of splicing factors commonly occur during other modes of transformation. We have further investigated the expression of five splicing regulatory factors, heterogeneous nuclear ribonucleoprotein (hnRNP) A1 and A2, alternative splicing factor/splicing factor 2 (ASF/SF2), spliceosome component of 35-kDa protein (SC35) and 65-kDa subunit of U2 snRNP auxiliary factor (U2AF65), using two series of clonally-derived mouse fibroblast cell lines, that were SV40 large T transfectants (SV-T2 and NIH3T3/13C7) or c-erbB2 transfectants (A4). U2AF65 expression was increased (2.1- and 2.7-fold) in NIH3T3/13C7 and A4 compared with the normal parental cells, respectively, and SC35 expression was increased 1.8- to 2.3-fold in all transformed cells. These results suggest that altered expression of some splicing regulatory factors may commonly occur during various modes of cellular transformation.

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May-June 2001
Volume 8 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Maeda T and Maeda T: Transformation-associated changes in gene expression of alternative splicing regulatory factors in mouse fibroblast cells. Oncol Rep 8: 563-566, 2001
APA
Maeda, T., & Maeda, T. (2001). Transformation-associated changes in gene expression of alternative splicing regulatory factors in mouse fibroblast cells. Oncology Reports, 8, 563-566. https://doi.org/10.3892/or.8.3.563
MLA
Maeda, T., Furukawa, S."Transformation-associated changes in gene expression of alternative splicing regulatory factors in mouse fibroblast cells". Oncology Reports 8.3 (2001): 563-566.
Chicago
Maeda, T., Furukawa, S."Transformation-associated changes in gene expression of alternative splicing regulatory factors in mouse fibroblast cells". Oncology Reports 8, no. 3 (2001): 563-566. https://doi.org/10.3892/or.8.3.563