Cycloprodigiosin hydrochloride, a H+/Cl- symporter, induces apoptosis in human colon cancer cell lines in vitro

Yamamoto,C.; Takemoto,H.; Kuno,K.; Yamamoto,D.; Nakai,K.; Baden,T.; Kamata,K.; Hirata,H.; Watanabe,T.; Inoue,K.

doi:10.3892/or.8.4.821

Cycloprodigiosin hydrochloride, a H+/Cl- symporter, induces apoptosis in human colon cancer cell lines in vitro

Authors:
- C. Yamamoto
- H. Takemoto
- K. Kuno
- D. Yamamoto
- K. Nakai
- T. Baden
- K. Kamata
- H. Hirata
- T. Watanabe
- K. Inoue
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Affiliations: Department of Internal Medicine III, Kansai Medical University, Moriguchi, Osaka 570-8506, Japan
Published online on: July 1, 2001 https://doi.org/10.3892/or.8.4.821
Pages: 821-824

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Abstract

Recently, we reported that cycloprodigiosin hydrochloride (cPrG·HCl), a novel H+/Cl- symporter, induces acidification of the cytosol and leads to apoptosis on rat and human liver cancer cells. In the present study, the effects of cPrG·HCl, a H+/Cl- symporter, were examined in colon cancer cell lines in vitro. In the MTT assay, cPrG·HCl inhibited the growth of two colon cancer cell lines (WiDr and SW480) in a dose- and time-dependent manner. The cPrG·HCl treatment of both types of cells induced apoptosis as confirmed by the appearance of a sub-G1 population and intranucleosomal DNA fragmentation. In addition, cPrG·HCl lowered pHi (below pH 6.8) respectively. Therefore, these results suggest that cPrG·HCl may be useful for the treatment of colon cancer cells.