The Das-1 immunostain is useful for discriminating metastatic colon adenocarcinoma from cholangiocarcinoma and hepatocellular carcinoma

  • Authors:
    • Robert L. Zimmerman
    • Kiron M. Das
    • Franz Fogt
    • Melissa Burke
    • Linda A. Murakata
  • View Affiliations

  • Published online on: November 1, 2002     https://doi.org/10.3892/or.9.6.1369
  • Pages: 1369-1372
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Abstract

The distinction between cholangiocarcinoma, hepatocellular carcinoma and liver metastasis is important but at times difficult solely on microscopic appearances. The Das-1 immunostain exhibits specificity for colonic epithelium. However, its staining of cholangiocarcinomas and hepatocellular carcinomas has not been extensively studied. We evaluated the staining properties of the Das-1 immunostain in cholangiocarcinoma and hepatocellular carcinoma. Forty-four cholangiocarcinomas, 16 hepatocellular carcinomas, 17 colon adenocarcinomas metastatic to the liver and 3 benign biliary tumors were studied. The cases were stained with the Das-1 antibody following deparaffinization and rehydration. The slides were evaluated for membranous and/or cytoplasmic staining in a blinded fashion. The percentage of tumor cells exhibiting strong staining was estimated. Of the intra-hepatic cholangiocarcinomas, 4 of 36 (11%) and 2 of 16 (13%) hepatocellular carcinomas were positive for Das-1, though typically only in rare cells. In comparison, 7 of 8 (88%) extra-hepatic cholangiocarcinomas, 13 of 17 (77%) of metastatic colon carcinomas and 3 of 3 (100%) benign tumors of the biliary tract were strongly positive in a diffuse pattern. The Das-1 immunostain may play a useful role in evaluating liver malignancies.

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November-December 2002
Volume 9 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zimmerman RL, Das KM, Fogt F, Burke M and Murakata LA: The Das-1 immunostain is useful for discriminating metastatic colon adenocarcinoma from cholangiocarcinoma and hepatocellular carcinoma. Oncol Rep 9: 1369-1372, 2002
APA
Zimmerman, R.L., Das, K.M., Fogt, F., Burke, M., & Murakata, L.A. (2002). The Das-1 immunostain is useful for discriminating metastatic colon adenocarcinoma from cholangiocarcinoma and hepatocellular carcinoma. Oncology Reports, 9, 1369-1372. https://doi.org/10.3892/or.9.6.1369
MLA
Zimmerman, R. L., Das, K. M., Fogt, F., Burke, M., Murakata, L. A."The Das-1 immunostain is useful for discriminating metastatic colon adenocarcinoma from cholangiocarcinoma and hepatocellular carcinoma". Oncology Reports 9.6 (2002): 1369-1372.
Chicago
Zimmerman, R. L., Das, K. M., Fogt, F., Burke, M., Murakata, L. A."The Das-1 immunostain is useful for discriminating metastatic colon adenocarcinoma from cholangiocarcinoma and hepatocellular carcinoma". Oncology Reports 9, no. 6 (2002): 1369-1372. https://doi.org/10.3892/or.9.6.1369