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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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August 2008 Volume 20 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

Transfection of the DAAO gene and subsequent induction of cytotoxic oxidative stress by D-alanine in 9L cells

  • Authors:
    • Jun Li
    • Yuechun Shen
    • Anzhong Liu
    • Xiaoming Wang
    • Chaochen Zhao

  • View Affiliations / Copyright

    Affiliations: Division of Hepatic Surgery, Department of Surgery, The First Affiliated Hospital, Guangzhou Medical College, Guangzhou 510120, P.R. China
  • Pages: 341-346
    |
    Published online on: August 1, 2008
       https://doi.org/10.3892/or_00000012
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Abstract

D-amino acid oxidase (DAAO) can catalyze the dehydrogenation of D-amino acids, such as D-alanine, to the corresponding amino acids and is then reoxidized by molecular oxygen to yield hydrogen peroxide, a reactive oxygen species, which reacts with DNA, lipids and protein, inducing cell death. This study investigated whether rat glioma 9L cells infected with the recombinant retrovirus containing the DAAO cDNA fragment can be induced in order to undergo cytotoxic oxidative stress by D-alanine. The recombinant retroviral vector, plzrus-DAAO-FLAG-GFP (pl-Dfg), was constructed and used to transfect packaged phoenix cells. The supernatant containing recombinant retroviral particles from the transfected phoenix cells was harvested and utilized to infect target 9L cells. The cytotoxic oxidative stress of infected 9L cells was induced by the DAAO substrate, D-alanine. The plasmid pl-Dfg was successfully constructed. The high titer retroviral supernatant was obtained from the transfected phoenix cells. Infected 9L cells were less viable after exposure to D-alanine compared to the control group. Anti-apoptotic proteins significantly inhibited cell death. The DAAO/D-alanine system has a potential utility for gene therapy and may be an effective strategy for the treatment of brain cancer and other malignant tumors.

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Copy and paste a formatted citation
Spandidos Publications style
Li J, Shen Y, Liu A, Wang X and Zhao C: Transfection of the DAAO gene and subsequent induction of cytotoxic oxidative stress by D-alanine in 9L cells. Oncol Rep 20: 341-346, 2008.
APA
Li, J., Shen, Y., Liu, A., Wang, X., & Zhao, C. (2008). Transfection of the DAAO gene and subsequent induction of cytotoxic oxidative stress by D-alanine in 9L cells. Oncology Reports, 20, 341-346. https://doi.org/10.3892/or_00000012
MLA
Li, J., Shen, Y., Liu, A., Wang, X., Zhao, C."Transfection of the DAAO gene and subsequent induction of cytotoxic oxidative stress by D-alanine in 9L cells". Oncology Reports 20.2 (2008): 341-346.
Chicago
Li, J., Shen, Y., Liu, A., Wang, X., Zhao, C."Transfection of the DAAO gene and subsequent induction of cytotoxic oxidative stress by D-alanine in 9L cells". Oncology Reports 20, no. 2 (2008): 341-346. https://doi.org/10.3892/or_00000012
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Shen Y, Liu A, Wang X and Zhao C: Transfection of the DAAO gene and subsequent induction of cytotoxic oxidative stress by D-alanine in 9L cells. Oncol Rep 20: 341-346, 2008.
APA
Li, J., Shen, Y., Liu, A., Wang, X., & Zhao, C. (2008). Transfection of the DAAO gene and subsequent induction of cytotoxic oxidative stress by D-alanine in 9L cells. Oncology Reports, 20, 341-346. https://doi.org/10.3892/or_00000012
MLA
Li, J., Shen, Y., Liu, A., Wang, X., Zhao, C."Transfection of the DAAO gene and subsequent induction of cytotoxic oxidative stress by D-alanine in 9L cells". Oncology Reports 20.2 (2008): 341-346.
Chicago
Li, J., Shen, Y., Liu, A., Wang, X., Zhao, C."Transfection of the DAAO gene and subsequent induction of cytotoxic oxidative stress by D-alanine in 9L cells". Oncology Reports 20, no. 2 (2008): 341-346. https://doi.org/10.3892/or_00000012
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