High corn oil and high extra virgin olive oil diets have different effects on the expression of differentiation-related genes in experimental mammary tumors

  • Authors:
    • Raquel Moral
    • Montserrat Solanas
    • Gemma Garcia
    • Laura Grau
    • Elena Vela
    • Raquel Escrich
    • Eduard Escrich
  • View Affiliations

  • Published online on: August 1, 2008     https://doi.org/10.3892/or_00000025
  • Pages: 429-435
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Abstract

Dietary lipids can modify the clinical behavior and morphological features of experimental breast tumors. We previously demonstrated that a high corn oil diet has a tumor-enhancing effect in 7,12-dimethylbenz(α)anthracene (DMBA)-induced rat mammary adenocarcinomas, whereas a high olive oil diet acts as a negative modulator of carcinogenesis. In this study, we investigated whether these high fat diets modulate the expression of genes related to differentiation. Rats were induced with DMBA and fed a low fat diet, a high corn oil diet, a high olive oil diet, or both high fat diets. The expression levels of the mammary differentiation biomarkers α-casein, β-casein and transferrin and of β-actin and its transporter zipcode binding protein 1 (ZBP1) were analyzed by Northern and/or Western blot in the mammary adenocarcinomas. The high fat diets did not induce changes in the expression of caseins, while transferrin expression was increased as a result of the high olive oil diet. β-actin mRNA levels were higher in the high fat diet groups, though no changes in the protein levels were observed. The expression of ZBP1, a protein reported as having a role in carcinogenesis, was significantly increased by the high corn oil diet. These results suggest that in this model caseins are not good biomarkers of the changes in tumor morphological differentiation conferred by the high fat diets. The modulation of transferrin and ZBP1 expression by the high olive oil and the high corn oil diets could be one of the mechanisms by which such diets have a different influence on mammary carcinogenesis.

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August 2008
Volume 20 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Moral R, Solanas M, Garcia G, Grau L, Vela E, Escrich R and Escrich E: High corn oil and high extra virgin olive oil diets have different effects on the expression of differentiation-related genes in experimental mammary tumors. Oncol Rep 20: 429-435, 2008.
APA
Moral, R., Solanas, M., Garcia, G., Grau, L., Vela, E., Escrich, R., & Escrich, E. (2008). High corn oil and high extra virgin olive oil diets have different effects on the expression of differentiation-related genes in experimental mammary tumors. Oncology Reports, 20, 429-435. https://doi.org/10.3892/or_00000025
MLA
Moral, R., Solanas, M., Garcia, G., Grau, L., Vela, E., Escrich, R., Escrich, E."High corn oil and high extra virgin olive oil diets have different effects on the expression of differentiation-related genes in experimental mammary tumors". Oncology Reports 20.2 (2008): 429-435.
Chicago
Moral, R., Solanas, M., Garcia, G., Grau, L., Vela, E., Escrich, R., Escrich, E."High corn oil and high extra virgin olive oil diets have different effects on the expression of differentiation-related genes in experimental mammary tumors". Oncology Reports 20, no. 2 (2008): 429-435. https://doi.org/10.3892/or_00000025