Significance of p-STAT3 expression in human colorectal adenocarcinoma

  • Authors:
    • Jae-Kyoung Park
    • Ran Hong
    • Kyung-Jong Kim
    • Tae-Bum Lee
    • Sung-Chul Lim
  • View Affiliations

  • Published online on: September 1, 2008     https://doi.org/10.3892/or_00000047
  • Pages: 597-604
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Abstract

To examine the relationship between p-STAT3 and the clinicopathological parameters of colorectal adenocarcinoma (CRA), we initially conducted immunohistochemical (IHC) analyses on formalin-fixed tissues. A total of 127 invasive CRA, 20 colorectal adenomas and 20 normal mucosae were obtained. To clarify the validity of p-STAT3 as determined by the IHC analysis, quantitative real-time PCR was performed on fresh samples from 51 CRA-4 carcinomas in situ, 47 invasive CRA and on 51 normal mucosae. IHC analyses were conducted after formalin fixation from 51 CRA for comparison. The statistically significant difference of immunoreactivity for p-STAT3 between the CRA and adenoma, and between the CRA and normal mucosae was identified. Among the 174 CRA, p-STAT3 immunoreactivity significantly correlated with the T- and clinical stage. Among the 47 invasive CRA, the expression of STAT3 as determined by real-time PCR significantly correlated with tumor size, M stage and clinical stage. The overall findings of the real-time PCR analyses correlated with the findings of the IHC analyses. These findings suggest that p-STAT3 expression has an important role related to the tumorigenesis and tumor progression of CRAs. Moreover, IHC analysis is a reliable and useful modality of assessing the status of p-STAT3 expression in formalin-fixed samples.

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September 2008
Volume 20 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Park J, Hong R, Kim K, Lee T and Lim S: Significance of p-STAT3 expression in human colorectal adenocarcinoma. Oncol Rep 20: 597-604, 2008
APA
Park, J., Hong, R., Kim, K., Lee, T., & Lim, S. (2008). Significance of p-STAT3 expression in human colorectal adenocarcinoma. Oncology Reports, 20, 597-604. https://doi.org/10.3892/or_00000047
MLA
Park, J., Hong, R., Kim, K., Lee, T., Lim, S."Significance of p-STAT3 expression in human colorectal adenocarcinoma". Oncology Reports 20.3 (2008): 597-604.
Chicago
Park, J., Hong, R., Kim, K., Lee, T., Lim, S."Significance of p-STAT3 expression in human colorectal adenocarcinoma". Oncology Reports 20, no. 3 (2008): 597-604. https://doi.org/10.3892/or_00000047