Stable suppression of HER-2 gene expression using siRNA increases the lysis of human ovarian carcinoma cells mediated by NK-92 cell line

  • Authors:
    • Ying Zhou
    • Zhixiang Cheng
    • Huaiping Zhu
    • Dingqing Feng
    • Weidong Zhao
    • Bin Ling
    • Haiming Wei
    • Zhigang Tian
  • View Affiliations

  • Published online on: December 1, 2008     https://doi.org/10.3892/or_00000162
  • Pages: 1425-1431
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Abstract

The overexpression and amplification of HER-2 gene is associated with the malignant biological behavior of ovarian carcinoma and these tumor cells expressing elevated levels of HER-2 appear to be resistant to the cytolysis of NK-92. In this study, we analyzed the cytolysis effects of NK-92 on human ovarian carcinoma cells (SK-OV-3) after inhibition the expression of HER-2 mRNA by siRNA. Human ovarian carcinoma cell line SK-OV-3 was transfected with siRNA-hairpin expression retroviral vector (HER-2/siRNA) designed to target HER-2 mRNA. A negative control was established utilizing a vector lacking the antisense component (HER-2/negative). The expression levels of HER-2 gene in SK-OV-3/siRNA, and SK-OV-3/negative cell lines were evaluated by semi-quantitative RT-PCR and immunohistochemistry. The growth and the early apoptosis of these cells were assayed by MTT and flow cytometry, respectively. The cytotoxicity of NK-92 against target cells was investigated by LDH. SK-OV-3/siRNA and SK-OV-3 cells were injected subcutaneously into BALB/c nude mice respectively and NK-92 cells were intraperitoneally injected to examine the anti-tumor activity in vivo. The stable cell line (SK-OV-3/siRNA) with a persistent silence of HER-2 was established. The inhibited expression of HER-2 gene was exhibited by semi-quantitative RT-PCR and immunohistochemistry. The suppressed proliferation and the elicitation of early apoptosis cells were observed in SK-OV-3/siRNA cell line. NK-92 cell line can efficiently lyse the SK-OV-3/siRNA cells in vitro and significantly inhibit the growth of tumors xenografted with SK-OV-3/siRNA cells. Suppression of HER-2 gene expression using siRNA combined treatment of NK-92 presents a new strategy for NK-92 biological treatment on the HER-2 expression epithelial tumors.

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December 2008
Volume 20 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhou Y, Cheng Z, Zhu H, Feng D, Zhao W, Ling B, Wei H and Tian Z: Stable suppression of HER-2 gene expression using siRNA increases the lysis of human ovarian carcinoma cells mediated by NK-92 cell line. Oncol Rep 20: 1425-1431, 2008
APA
Zhou, Y., Cheng, Z., Zhu, H., Feng, D., Zhao, W., Ling, B. ... Tian, Z. (2008). Stable suppression of HER-2 gene expression using siRNA increases the lysis of human ovarian carcinoma cells mediated by NK-92 cell line. Oncology Reports, 20, 1425-1431. https://doi.org/10.3892/or_00000162
MLA
Zhou, Y., Cheng, Z., Zhu, H., Feng, D., Zhao, W., Ling, B., Wei, H., Tian, Z."Stable suppression of HER-2 gene expression using siRNA increases the lysis of human ovarian carcinoma cells mediated by NK-92 cell line". Oncology Reports 20.6 (2008): 1425-1431.
Chicago
Zhou, Y., Cheng, Z., Zhu, H., Feng, D., Zhao, W., Ling, B., Wei, H., Tian, Z."Stable suppression of HER-2 gene expression using siRNA increases the lysis of human ovarian carcinoma cells mediated by NK-92 cell line". Oncology Reports 20, no. 6 (2008): 1425-1431. https://doi.org/10.3892/or_00000162