Decrease in malonyl-CoA and its background metabolic alterations in murine model of cancer cachexia

  • Authors:
    • Alper Celik
    • Yoshihiko Kano
    • Shingo Tsujinaka
    • Sinichirou Okada
    • Koichi Takao
    • Masakazu Takagi
    • Shigeru Chohnan
    • Kuniyasu Soda
    • Masanobu Kawakami
    • Fumio Konishi
  • View Affiliations

  • Published online on: April 1, 2009     https://doi.org/10.3892/or_00000330
  • Pages: 1105-1111
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Abstract

The alterations of enzymatic activities involved in lipid degradation in cancer cachexia have not been fully elucidated. One of the two subclones of colon 26 adenocarcinoma, clone 20, with a potent ability to induce cachexia, or clone 5, without such an activity, was transplanted in to CDF-1 male mice. Murine livers were extirpated for analyses on the 14th day after tumor inoculation. The body weights and food intake of mice bearing clone 20 were all significantly lower than those of non-tumor bearing mice and mice bearing the clone 5 tumor. The decline of body weight was accompanied by a shrinkage of epididymal fat pads. Expression of spermidine/spermine N-1 acetyl transferase (SSAT) assessed by real-time PCR was significantly increased in cachectic mice. Conversely, acetyl-CoA carboxylase (ACC) measured by Western blotting and malonyl-CoA levels determined by malonyl-CoA:acetyl-CoA cycling procedures were decreased in cachectic mice. Indomethacin in drinking water reversed the clone 20 induced decrease in body and fat weight and food intake, and simultaneously negated the clone 20 induced increase of SSAT expressions and decrease of ACC and malonyl-CoA amounts. Because malonyl-CoA inhibits the rate-limiting step in the beta-oxidation of fatty acids, the decreased malonyl-CoA and the background metabolic alterations may contribute to the accelerated lipolysis of cancer cachexia.

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April 2009
Volume 21 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Celik A, Kano Y, Tsujinaka S, Okada S, Takao K, Takagi M, Chohnan S, Soda K, Kawakami M, Konishi F, Konishi F, et al: Decrease in malonyl-CoA and its background metabolic alterations in murine model of cancer cachexia. Oncol Rep 21: 1105-1111, 2009
APA
Celik, A., Kano, Y., Tsujinaka, S., Okada, S., Takao, K., Takagi, M. ... Konishi, F. (2009). Decrease in malonyl-CoA and its background metabolic alterations in murine model of cancer cachexia. Oncology Reports, 21, 1105-1111. https://doi.org/10.3892/or_00000330
MLA
Celik, A., Kano, Y., Tsujinaka, S., Okada, S., Takao, K., Takagi, M., Chohnan, S., Soda, K., Kawakami, M., Konishi, F."Decrease in malonyl-CoA and its background metabolic alterations in murine model of cancer cachexia". Oncology Reports 21.4 (2009): 1105-1111.
Chicago
Celik, A., Kano, Y., Tsujinaka, S., Okada, S., Takao, K., Takagi, M., Chohnan, S., Soda, K., Kawakami, M., Konishi, F."Decrease in malonyl-CoA and its background metabolic alterations in murine model of cancer cachexia". Oncology Reports 21, no. 4 (2009): 1105-1111. https://doi.org/10.3892/or_00000330