Effect of p27 on motility of MDA-MB-231 breast cancer cells

  • Authors:
    • Eun Young Lee
    • Min Jeong Jeon
    • Wonseok Yang
    • Incheol Shin
  • View Affiliations

  • Published online on: June 1, 2009     https://doi.org/10.3892/or_00000396
  • Pages: 1621-1625
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Abstract

p27 is a member of the Kip family of cyclin-dependent kinase inhibitors and its overexpression results in cell cycle arrest at G1 and/or apoptosis. In addition to its role as a regulator of cell cycle progression, p27 can also participate in cell motility, especially when it is mislocalized in the cytosol. To further elucidate the role of p27 in the motility of MDA-MB-231 breast cancer cells, we performed p27 knockdown in MDA-MB-231 cells by RNA interference. Infection of MDA-MB-231 cells with retroviruses harboring p27 short hairpin RNA (shRNA) designed from human p27 cDNA resulted in efficient inhibition of p27 expression, while p27 shRNA designed from mouse p27 cDNA did not affect p27 expression in MDA-MB-231 cells. MDA-MB-231 cells infected with human p27 shRNA (MDA-MB-231 hp27shRNA) showed increased proliferation compared to control MDA-MB-231 cells and MDA-MB-231 cells infected with mouse p27shRNA (MDA-MB-231 mp27shRNA). Wound healing assays revealed that migration of MDA-MB-231 hpshRNA cells was markedly impaired compared to MDA-MB-231 mpshRNA cells, especially when cycloheximide was added to block protein synthesis. Immunostaining of p27 in MDA-MB-231 cells showed that p27 predominantly localized in the nuclei. These results suggest that both nuclear and cytosolic p27 can promote cancer cell motility.

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June 2009
Volume 21 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Lee EY, Jeon MJ, Yang W and Shin I: Effect of p27 on motility of MDA-MB-231 breast cancer cells. Oncol Rep 21: 1621-1625, 2009
APA
Lee, E.Y., Jeon, M.J., Yang, W., & Shin, I. (2009). Effect of p27 on motility of MDA-MB-231 breast cancer cells. Oncology Reports, 21, 1621-1625. https://doi.org/10.3892/or_00000396
MLA
Lee, E. Y., Jeon, M. J., Yang, W., Shin, I."Effect of p27 on motility of MDA-MB-231 breast cancer cells". Oncology Reports 21.6 (2009): 1621-1625.
Chicago
Lee, E. Y., Jeon, M. J., Yang, W., Shin, I."Effect of p27 on motility of MDA-MB-231 breast cancer cells". Oncology Reports 21, no. 6 (2009): 1621-1625. https://doi.org/10.3892/or_00000396