Detection of glypican-3-specific CTLs in chronic hepatitis and liver cirrhosis

Hayashi,Emiko; Motomura,Yutaka; Shirakawa,Hirofumi; Yoshikawa,Toshiaki; Oba,Nobuyuki; Nishinakagawa,Shuta; Mizuguchi,Yasuhiro; Kojima,Tatsuya; Nomura,Kazuhiro; Nakatsura,Tetsuya

doi:10.3892/or_00000418

July 2009 Volume 22 Issue 1

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July 2009 Volume 22 Issue 1

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Article

Detection of glypican-3-specific CTLs in chronic hepatitis and liver cirrhosis

Authors:
- Emiko Hayashi
- Yutaka Motomura
- Hirofumi Shirakawa
- Toshiaki Yoshikawa
- Nobuyuki Oba
- Shuta Nishinakagawa
- Yasuhiro Mizuguchi
- Tatsuya Kojima
- Kazuhiro Nomura
- Tetsuya Nakatsura
View Affiliations / Copyright

Affiliations: Section for Cancer Immunotherapy, Investigative Treatment Division, Research Center for Innovative Oncology, National Cancer Center Hospital East, Chiba 277-8577, Japan. ehayashi@east.ncc.go.jp
Pages: 149-154
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Published online on: July 1, 2009

https://doi.org/10.3892/or_00000418
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Abstract

Glypican-3 (GPC3) is one of carcinoembryonic antigens known to be overexpressed in hepatocellular carcinoma (HCC). It has been suggested that GPC3 may be related to the development of HCC in a background of chronic hepatitis (CH) and liver cirrhosis (LC). Therefore, in an attempt to establish an early diagnostic marker of HCC, we quantified the number of GPC3-specific CTLs in the peripheral blood of CH and LC patients. We selected CH and LC patients who were HCV-RNA (+) or HBs antigen (+) within 6 months prior to the study and had no HCC nodules as detected by imaging. A total of 56 patients with CH and LC, and 45 patients with HLA-A24+ or HLA-A2+ were enrolled for this investigation. After isolation of mononuclear cells from each patient's peripheral blood specimens, we performed ELISPOT assay using HLA-A24- and HLA-A2-restricted GPC3 peptides. In the ELISPOT assay, GPC3-specific CTLs were detected in 10 of the 45 CH and LC cases (22%). In addition, the plasma titers of anti-GPC3 IgG were increased in the CH and LC patients as compared with those in healthy donors. GPC3-specific CTLs were found to be present not only in patients with HCC, but also in patients with CH and LC. This suggests the possibility of GPC3-specific CTLs serving as a marker for the early diagnosis of imaging-invisible HCC.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Detection of glypican-3-specific CTLs in chronic hepatitis and liver cirrhosis

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Abstract

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