Susceptibility of the human LNCaP prostate cancer cells to the apoptotic effect of marina crystal minerals (MCM) in vitro

  • Authors:
    • Mamdooh Ghoneum
    • Sastry Gollapudi
  • View Affiliations

  • Published online on: July 1, 2009     https://doi.org/10.3892/or_00000419
  • Pages: 155-159
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Abstract

Prostate cancer is one of the most frequent cancers among men in the United States. In the current study, we examined the susceptibility of the human LNCaP prostate cancer cells to the apoptotic effect of marina crystal minerals (MCM), a crystallized mixture of minerals and trace elements from sea water, in vitro. Cancer cells were cultured with MCM at different concentrations (0-1000 µg/ml). At day 3, MCM exhibited a marked apoptotic effect on LNCaP cells as measured by propidium iodide (PI) and Giemsa-stained cytospin preparations. The apoptotic effect was dose-dependent: a 2-fold increase in the level of apoptosis over the control cells was observed at low concentrations of 50 µg/ml, maximial (3-fold) at a concentration of 500-1000 µg/ml. The mechanism by which MCM induces apoptosis was examined. Flow cytometery analysis demonstrated that mitochondrial membrane potential was significantly decreased post-treatment with MCM at low concentrations of 100 µg/ml. In addition, Western blot analysis showed that the level of anti-apoptotic molecule Bcl-2 in LNCaP cells decreased upon treatment with MCM. We conclude that MCM has an apoptotic effect toward human prostate cancer cells, which may suggest its broader potential as an innovative, non-toxic anti-cancer agent.

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July 2009
Volume 22 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ghoneum M and Ghoneum M: Susceptibility of the human LNCaP prostate cancer cells to the apoptotic effect of marina crystal minerals (MCM) in vitro. Oncol Rep 22: 155-159, 2009
APA
Ghoneum, M., & Ghoneum, M. (2009). Susceptibility of the human LNCaP prostate cancer cells to the apoptotic effect of marina crystal minerals (MCM) in vitro. Oncology Reports, 22, 155-159. https://doi.org/10.3892/or_00000419
MLA
Ghoneum, M., Gollapudi, S."Susceptibility of the human LNCaP prostate cancer cells to the apoptotic effect of marina crystal minerals (MCM) in vitro". Oncology Reports 22.1 (2009): 155-159.
Chicago
Ghoneum, M., Gollapudi, S."Susceptibility of the human LNCaP prostate cancer cells to the apoptotic effect of marina crystal minerals (MCM) in vitro". Oncology Reports 22, no. 1 (2009): 155-159. https://doi.org/10.3892/or_00000419