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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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August 2009 Volume 22 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Differences in expression between transcripts using alternative promoters of hMLH1 gene and their correlation with microsatellite instability

  • Authors:
    • Ryosuke Takahashi
    • Keiichi Nagai

  • View Affiliations / Copyright

    Affiliations: Advanced Research Laboratory, Hitachi, Ltd., Saitama 350-0395, Japan
  • Pages: 265-271
    |
    Published online on: August 1, 2009
       https://doi.org/10.3892/or_00000433
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Abstract

hMLH1 is involved in DNA mismatch repair and its defects cause hereditary non-polyposis colorectal cancer (HNPCC) as well as other types of cancer. A defective DNA mismatch repair system results in genetic instability, also referred to as microsatellite instability (MSI), which is a good indicator of HNPCC. Using in silico analysis of oligo-capping cDNA sequences, we initially identified a splicing (variant type 2) whose second exon is 5 bp shorter than that of a genuine hMLH1 transcript (variant type 1) and a transcript using alternative promoter (variant type 3) whose transcription starts about 300 bases downstream of variant type 1. We then compared the expression level of variant types 1 and 3 among six colorectal cancer cell lines using real-time PCR. As a result, we found that the cell lines that completely suppress the expression of variant type 1 by hypermethylation expressed variant type 3 to a certain extent. This result suggests that the expression of variant types 1 and 3 did not completely follow the same transcription mechanism. We also found that the cell lines showing MSI to be positive either expressed variant type 3 more than type 1 or expressed only variant type 3. These results showed the potential applicability of mRNA expression analysis to molecular diagnostic tests of MSI-positive cancer types.

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Copy and paste a formatted citation
Spandidos Publications style
Takahashi R and Nagai K: Differences in expression between transcripts using alternative promoters of hMLH1 gene and their correlation with microsatellite instability. Oncol Rep 22: 265-271, 2009.
APA
Takahashi, R., & Nagai, K. (2009). Differences in expression between transcripts using alternative promoters of hMLH1 gene and their correlation with microsatellite instability. Oncology Reports, 22, 265-271. https://doi.org/10.3892/or_00000433
MLA
Takahashi, R., Nagai, K."Differences in expression between transcripts using alternative promoters of hMLH1 gene and their correlation with microsatellite instability". Oncology Reports 22.2 (2009): 265-271.
Chicago
Takahashi, R., Nagai, K."Differences in expression between transcripts using alternative promoters of hMLH1 gene and their correlation with microsatellite instability". Oncology Reports 22, no. 2 (2009): 265-271. https://doi.org/10.3892/or_00000433
Copy and paste a formatted citation
x
Spandidos Publications style
Takahashi R and Nagai K: Differences in expression between transcripts using alternative promoters of hMLH1 gene and their correlation with microsatellite instability. Oncol Rep 22: 265-271, 2009.
APA
Takahashi, R., & Nagai, K. (2009). Differences in expression between transcripts using alternative promoters of hMLH1 gene and their correlation with microsatellite instability. Oncology Reports, 22, 265-271. https://doi.org/10.3892/or_00000433
MLA
Takahashi, R., Nagai, K."Differences in expression between transcripts using alternative promoters of hMLH1 gene and their correlation with microsatellite instability". Oncology Reports 22.2 (2009): 265-271.
Chicago
Takahashi, R., Nagai, K."Differences in expression between transcripts using alternative promoters of hMLH1 gene and their correlation with microsatellite instability". Oncology Reports 22, no. 2 (2009): 265-271. https://doi.org/10.3892/or_00000433
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