Tubulin inhibitor AEZS 112 inhibits the growth of experimental human ovarian and endometrial cancers irrespective of caspase inhibition

  • Authors:
    • Jörg B. Engel
    • Tanja Schönhals
    • Claudia Weidler
    • Sebastian Häusler
    • Mathias Krockenberger
    • Lorenz Rieger
    • Johannes Dietl
    • Jörg Wischhusen
    • Arnd Honig
  • View Affiliations

  • Published online on: August 1, 2009     https://doi.org/10.3892/or_00000446
  • Pages: 361-367
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Abstract

AEZS 112 is an orally active small molecule anticancer drug which inhibits the polymerization of tubulin at low micromolar concentrations. The current study investigates the anti-tumor effect and the mechanism of action of AEZS 112 in in vitro models of human ovarian and endometrial cancers. Four human ovarian and 2 endometrial cancer cell lines were incubated with increasing concentrations of AEZS 112 with and without multi-caspase inhibitor zVAD-FMK for 72 hours. Cytotoxic effects of AEZS 112 were analyzed using crystal violet staining, FACS analysis of DNA content as well as Annexin V/propidium iodide-double staining. AEZS 112 displayed anti-tumor activity in all six cell lines. The EC50 determined after 72-h incubation for Ishikawa and HEC 1A was 0.0312 and 0.125 µm, respectively. The EC50 was 5 µm for SKOV 3 cells, 1 µm for 0.5 µm for OAW 42 cells, 0.125 µm for OvW 1 cells and 0.0312 µm for PA 1 cells. Cytotoxic effects of AEZS 112 could not be abrogated by caspase inhibition with pan-caspase inhibitor zVAD-fmk. Annexin V/propidium iodide-double staining after treatment with AEZS 112 was indicative of necrosis-like cell death. AEZS 112 dose-dependently increased non-vital hypodiploid cells and the cytotoxic effect was least pronounced in G2 phase of the cell cycle, indicating cell death during mitosis, as determined by FACS analysis. The orally active small molecule tubulin inhibitor AEZS 112 showed anti-tumor activity in human ovarian and endometrial cancer cell lines at low micromolar concentrations, which could not be abrogated by caspase inhibition and is therefore a good candidate for in vivo studies in these tumors.

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August 2009
Volume 22 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Engel JB, Schönhals T, Weidler C, Häusler S, Krockenberger M, Rieger L, Dietl J, Wischhusen J and Honig A: Tubulin inhibitor AEZS 112 inhibits the growth of experimental human ovarian and endometrial cancers irrespective of caspase inhibition. Oncol Rep 22: 361-367, 2009
APA
Engel, J.B., Schönhals, T., Weidler, C., Häusler, S., Krockenberger, M., Rieger, L. ... Honig, A. (2009). Tubulin inhibitor AEZS 112 inhibits the growth of experimental human ovarian and endometrial cancers irrespective of caspase inhibition. Oncology Reports, 22, 361-367. https://doi.org/10.3892/or_00000446
MLA
Engel, J. B., Schönhals, T., Weidler, C., Häusler, S., Krockenberger, M., Rieger, L., Dietl, J., Wischhusen, J., Honig, A."Tubulin inhibitor AEZS 112 inhibits the growth of experimental human ovarian and endometrial cancers irrespective of caspase inhibition". Oncology Reports 22.2 (2009): 361-367.
Chicago
Engel, J. B., Schönhals, T., Weidler, C., Häusler, S., Krockenberger, M., Rieger, L., Dietl, J., Wischhusen, J., Honig, A."Tubulin inhibitor AEZS 112 inhibits the growth of experimental human ovarian and endometrial cancers irrespective of caspase inhibition". Oncology Reports 22, no. 2 (2009): 361-367. https://doi.org/10.3892/or_00000446