Enhanced radiosensitivity of SW480 cells via TRAIL up-regulation mediated by Egr-1 promoter

  • Authors:
    • Y. Hu
    • W. Ouyang
    • F. Wu
    • C. H. Cao
    • K. Wang
    • Z. K. Liao
    • Y. H. Zhong
    • F. X. Zhou
    • S. Q. Liu
    • L. Xia
    • Y. F. Zhou
    • C. H. Xie
  • View Affiliations

  • Published online on: October 1, 2009     https://doi.org/10.3892/or_00000498
  • Pages: 765-771
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Abstract

Radiosensitization of cancer cells to irradiation could improve the efficacy of radiotherapy. The early transcriptional factor (Egr-1) promoter induced expression of downstream genes after irradiation. TNF-related apoptosis-inducing ligand (TRAIL) is known to induce apoptosis in malignant cells, but displayed little or no toxicity on normal cells. In this study, we constructed pcDNA3.1-Egr-1-TRAIL (pEgr.1-TRAIL) recombinant plasmid and evaluated its effect on human colon cancer cell line SW480. pEgr.1-TRAIL transfection combined with radiotherapy caused dramatically elevation of TRAIL expression both in mRNA and protein levels, much lower radiobiological parameters in clonogenic assays, accompanied by remarkably increase in apoptosis ratio. Furthermore, pEgr.1-TRAIL transfected cells displayed higher proportion in G0/G1 phase. Our results suggested that pEgr.1-TRAIL can sensitize SW480 cells to radiation, and the radiosensitization is related to cell cycle changes and apoptosis mediated by up-regulation of TRAIL expression. These findings support the potential future application of genetic radiotherapy against carcinoma.

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October 2009
Volume 22 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Hu Y, Ouyang W, Wu F, Cao CH, Wang K, Liao ZK, Zhong YH, Zhou FX, Liu SQ, Xia L, Xia L, et al: Enhanced radiosensitivity of SW480 cells via TRAIL up-regulation mediated by Egr-1 promoter. Oncol Rep 22: 765-771, 2009
APA
Hu, Y., Ouyang, W., Wu, F., Cao, C.H., Wang, K., Liao, Z.K. ... Xie, C.H. (2009). Enhanced radiosensitivity of SW480 cells via TRAIL up-regulation mediated by Egr-1 promoter. Oncology Reports, 22, 765-771. https://doi.org/10.3892/or_00000498
MLA
Hu, Y., Ouyang, W., Wu, F., Cao, C. H., Wang, K., Liao, Z. K., Zhong, Y. H., Zhou, F. X., Liu, S. Q., Xia, L., Zhou, Y. F., Xie, C. H."Enhanced radiosensitivity of SW480 cells via TRAIL up-regulation mediated by Egr-1 promoter". Oncology Reports 22.4 (2009): 765-771.
Chicago
Hu, Y., Ouyang, W., Wu, F., Cao, C. H., Wang, K., Liao, Z. K., Zhong, Y. H., Zhou, F. X., Liu, S. Q., Xia, L., Zhou, Y. F., Xie, C. H."Enhanced radiosensitivity of SW480 cells via TRAIL up-regulation mediated by Egr-1 promoter". Oncology Reports 22, no. 4 (2009): 765-771. https://doi.org/10.3892/or_00000498