Efficient preparation of highly pure chlorin e6 and its photodynamic anti-cancer activity in a rat tumor model

  • Authors:
    • Yeon-Hee Moon
    • Seong-Min Kwon
    • Hyo-Jun Kim
    • Kwan-Young Jung
    • Jong-Hwan Park
    • Soo-A Kim
    • Yong-Chul Kim
    • Sang-Gun Ahn
    • Jung-Hoon Yoon
  • View Affiliations

  • Published online on: November 1, 2009     https://doi.org/10.3892/or_00000540
  • Pages: 1085-1091
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Abstract

Photodynamic therapy (PDT) is currently being used as an alternative therapeutic modality for a variety of malignant tumors. This study was performed to show an efficient preparation of second generation of photosensitizer chlorin e6 (Ce6) with high yield and purity, and to test antitumor activity of Ce6-induced PDT (Ce6-PDT) both in vitro and in vivo using a rat tumor model. Three-week-old male Sprague-Dawley (SD) rats were inoculated s.c. on the right flank with 5x106 RK3E-ras cells. The animals were admin-istered i.v. with Ce6 (10 mg/kg) and 24 h later, PDT was performed using a laser diode at a light dose of 100 J/cm2. Ce6-PDT generated reactive oxygen species and led to significant growth inhibition in RK3E-ras cell. In addition, Ce6-PDT induced apoptosis through the activation of caspase-3 and its downstream target, PARP cleavage. The protein level of anti-apoptotic bcl-2 was also reduced by Ce6-PDT in RK3E-ras cells. In in vivo experiments, application of Ce6-PDT led to a significant reduction of tumor size. PCNA immunostaining and TUNEL assay revealed that Ce6-PDT inhibited tumor cell proliferation and increased apoptosis. These findings suggest that the newly purified Ce6-PDT can effectively arrest tumor growth by inhibiting cell proliferation and inducing apoptosis.

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November 2009
Volume 22 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Moon Y, Kwon S, Kim H, Jung K, Park J, Kim S, Kim Y, Ahn S and Yoon J: Efficient preparation of highly pure chlorin e6 and its photodynamic anti-cancer activity in a rat tumor model. Oncol Rep 22: 1085-1091, 2009
APA
Moon, Y., Kwon, S., Kim, H., Jung, K., Park, J., Kim, S. ... Yoon, J. (2009). Efficient preparation of highly pure chlorin e6 and its photodynamic anti-cancer activity in a rat tumor model. Oncology Reports, 22, 1085-1091. https://doi.org/10.3892/or_00000540
MLA
Moon, Y., Kwon, S., Kim, H., Jung, K., Park, J., Kim, S., Kim, Y., Ahn, S., Yoon, J."Efficient preparation of highly pure chlorin e6 and its photodynamic anti-cancer activity in a rat tumor model". Oncology Reports 22.5 (2009): 1085-1091.
Chicago
Moon, Y., Kwon, S., Kim, H., Jung, K., Park, J., Kim, S., Kim, Y., Ahn, S., Yoon, J."Efficient preparation of highly pure chlorin e6 and its photodynamic anti-cancer activity in a rat tumor model". Oncology Reports 22, no. 5 (2009): 1085-1091. https://doi.org/10.3892/or_00000540