Molecular and immunological aspects of p53 and p53-autoantibodies in head and neck squamous cell carcinoma

  • Authors:
    • Christian Cordes
    • J. Von Lingen
    • T. Görögh
    • P. Ambrosch
    • S. Gottschlich
    • M. Hoffmann
  • View Affiliations

  • Published online on: December 1, 2009     https://doi.org/10.3892/or_00000568
  • Pages: 1299-1303
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Abstract

The tumour suppressor protein p53 (wild-type = wt-p53) is of major importance in the genetic integrity of the cell. Mutations of the p53-gene (mt-p53) are the most frequent genetic aberrations identified in different tumour entities. As analyzed in a wide variety of human malignomas, mt-p53 evokes a specific immune response. Yet, the possible occurrence of p53-autoantibodies in patients with head and neck squamous cell carcinomas (HNSCC) correlated to p53-mutations, p53 in sera and p53-overexpression in tissue has not been previously investigated. For the first time, the p53 status in 24 HNSCC patients was analyzed in the present study. The following parameters were investigated: analysis of mutation frequency of the p53-gene by direct sequencing of the exons 5-9, immunohistochemical detection of p53, measurement of the wt- and mt-p53-protein in sera by ELISA and p53-autoantibodies in sera by ELISA. Mutations of the p53-gene were detected in four (17%) patients. Overexpression of wt-p53 was detected by immunohistochemistry in 18 out of 24 (75%) tumours. In 8 (33%) patients the p53-protein was also detectable in sera, whereas in just one of these eight patients p53-autoantibodies were detectable simultaneously. Overall 6 out of 24 (25%) patients were found to be positive for serum p53-autoantibodies. Of these 6 cases, 5 could be assigned to tumours with immunohistochemically measurable wt-p53-overexpression. There was no correlation between p53-overexpression in tissue and p53-protein levels in sera or between p53-autoantibody levels in sera, nor in mutation frequency of the p53-gene and p53-overexpression in tissue. The results presented herein support the hypothesis that strong accumulation of p53 in the tissue is an important prerequisite for development of p53-autoantibodies. However, there must be further, yet unknown factors that influence the p53-autoantibody production because p53-autoantibodies were not identified in sera in each case of p53-accumulation in the tissue.

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December 2009
Volume 22 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Cordes C, Von Lingen J, Görögh T, Ambrosch P, Gottschlich S and Hoffmann M: Molecular and immunological aspects of p53 and p53-autoantibodies in head and neck squamous cell carcinoma. Oncol Rep 22: 1299-1303, 2009
APA
Cordes, C., Von Lingen, J., Görögh, T., Ambrosch, P., Gottschlich, S., & Hoffmann, M. (2009). Molecular and immunological aspects of p53 and p53-autoantibodies in head and neck squamous cell carcinoma. Oncology Reports, 22, 1299-1303. https://doi.org/10.3892/or_00000568
MLA
Cordes, C., Von Lingen, J., Görögh, T., Ambrosch, P., Gottschlich, S., Hoffmann, M."Molecular and immunological aspects of p53 and p53-autoantibodies in head and neck squamous cell carcinoma". Oncology Reports 22.6 (2009): 1299-1303.
Chicago
Cordes, C., Von Lingen, J., Görögh, T., Ambrosch, P., Gottschlich, S., Hoffmann, M."Molecular and immunological aspects of p53 and p53-autoantibodies in head and neck squamous cell carcinoma". Oncology Reports 22, no. 6 (2009): 1299-1303. https://doi.org/10.3892/or_00000568