Induction of matrix metalloproteinase-9 by galectin-7 through p38 MAPK signaling in HeLa human cervical epithelial adenocarcinoma cells

Park,Ji E.; Chang,Weon Y.; Cho,Moonjae

doi:10.3892/or_00000577

December 2009 Volume 22 Issue 6

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

December 2009 Volume 22 Issue 6

Full Size Image

Article

Induction of matrix metalloproteinase-9 by galectin-7 through p38 MAPK signaling in HeLa human cervical epithelial adenocarcinoma cells

Authors:
- Ji E. Park
- Weon Y. Chang
- Moonjae Cho
View Affiliations / Copyright

Affiliations: Department of Biochemistry, Cheju National University, Jeju 690-756, Korea
Pages: 1373-1379
|
Published online on: December 1, 2009

https://doi.org/10.3892/or_00000577
Expand metrics +

Abstract

The expression of matrix metalloproteinase-9 (MMP-9) has been reported in various cancers. Its expression is associated with tumorigenesis and tumor metastasis. Studies have shown that galectin-7, a β-galactoside-binding animal lectin, is involved in various processes including the suppression of tumor growth. However, a recent study reported that the development of thymic lymphoma is accelerated by galectin-7 expression. In this report, we demonstrate that the expression of MMP-9 was increased by galectin-7 in human cervix epithelial cells (HeLa). When the galectin-7 gene was transfected to HeLa cells with the ECFP vector, the expression of MMP-9 mRNA increased, as compared to non-transfected cells. As a result, MMP-9 protein levels also increased, as indicated by Western blot and gelatin zymography. In addition, galectin-7-transfected cells exhibited increased invasion in the matrigel invasion system. Expression of MMP-9 is involved in several signaling pathways by various stimulation factors. Therefore, we investigated how the signaling pathway in galectin-7-transfected cells differs from that of non-transfected cells. Upon transfection of galectin-7, p38 MAPK was activated and SB203580, a chemical inhibitor of p38 MAPK, reversed the effects of galectin-7. These results indicate that galectin-7 increases the expression of MMP-9 through the p38 MAPK signaling pathway.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Induction of matrix metalloproteinase-9 by galectin-7 through p38 MAPK signaling in HeLa human cervical epithelial adenocarcinoma cells

This article is mentioned in:

Abstract

Related Articles