Wnt-5a regulates proliferation in lung cancer cells

  • Authors:
    • Ying Huang
    • Guangxiang Liu
    • Bo Zhang
    • Gang Xu
    • Wei Xiong
    • Heping Yang
  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/or_00000620
  • Pages: 177-181
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Abstract

Wnt-5a is important in the physiological development and differentiation of lung and is also involved in the regulating proliferation, differentiation and invasion of tumor cells. However, very little is known about the roles of Wnt-5a in the development of lung cancer. The purpose of this study was to explore the role of Wnt-5a in the development of the non-small cell lung cancer, through constructing plasmids containing Wnt-5a sense gene siRNA. We constructed the plasmids containing the Wnt-5a sense gene and siRNA eukaryotic expression vector and transfected it into the human lung squamous carcinoma cell line H157 and adenocarcinoma cell line A549. Expression of Wnt-5a RNA and protein and proliferation of the cells were measured. Expression of Wnt-5a protein significantly stimulated cell proliferation and transfection with siRNA plasmids suppressed Wnt-5 expression and cell proliferation. The human plasmids containing Wnt-5a sense gene and siRNA eukaryotic expression vector was successfully constructed and transfection to human cancer cells induces cell proliferation. siRNA actively suppressed Wnt-5a expression.

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January 2010
Volume 23 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Huang Y, Liu G, Zhang B, Xu G, Xiong W and Yang H: Wnt-5a regulates proliferation in lung cancer cells. Oncol Rep 23: 177-181, 2010
APA
Huang, Y., Liu, G., Zhang, B., Xu, G., Xiong, W., & Yang, H. (2010). Wnt-5a regulates proliferation in lung cancer cells. Oncology Reports, 23, 177-181. https://doi.org/10.3892/or_00000620
MLA
Huang, Y., Liu, G., Zhang, B., Xu, G., Xiong, W., Yang, H."Wnt-5a regulates proliferation in lung cancer cells". Oncology Reports 23.1 (2010): 177-181.
Chicago
Huang, Y., Liu, G., Zhang, B., Xu, G., Xiong, W., Yang, H."Wnt-5a regulates proliferation in lung cancer cells". Oncology Reports 23, no. 1 (2010): 177-181. https://doi.org/10.3892/or_00000620