Preparation of a novel adenovirus formulation with artificial envelope of multilayer polymer-coatings: Therapeutic effect on metastatic ovarian cancer

  • Authors:
    • Chieko Yoshihara
    • Katsuyuki Hamada
    • Yoshiyuki Koyama
  • View Affiliations

  • Published online on: March 1, 2010     https://doi.org/10.3892/or_00000691
  • Pages: 733-738
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Abstract

Layer-by-layer deposition of the ionic polymers onto adenovirus particles afforded the multilayer-coated virus vectors. The infectivity of the virus in the presence of anti-adenovirus antibody increased as the layer number and the viruses with five or six polymer layers allowed relatively high efficiency of reporter gene expression in vitro. Therapeutic effect of the intraperitoneal injection of the oncolytic adenovirus with quintal polymer multilayers on the mice bearing intraperitoneal metastatic ovarian cancer was examined. All the control mice injected with PBS died within 21 days after the tumor inoculation. On the other hand, the mice injected with the multilayer-coated oncolytic virus lived much longer and seven eighths of them lived >60 days without apparent accumulation of ascites. These approaches would open a new way to create a novel, safe and efficient viral gene therapy.

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March 2010
Volume 23 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yoshihara C, Hamada K and Koyama Y: Preparation of a novel adenovirus formulation with artificial envelope of multilayer polymer-coatings: Therapeutic effect on metastatic ovarian cancer. Oncol Rep 23: 733-738, 2010.
APA
Yoshihara, C., Hamada, K., & Koyama, Y. (2010). Preparation of a novel adenovirus formulation with artificial envelope of multilayer polymer-coatings: Therapeutic effect on metastatic ovarian cancer. Oncology Reports, 23, 733-738. https://doi.org/10.3892/or_00000691
MLA
Yoshihara, C., Hamada, K., Koyama, Y."Preparation of a novel adenovirus formulation with artificial envelope of multilayer polymer-coatings: Therapeutic effect on metastatic ovarian cancer". Oncology Reports 23.3 (2010): 733-738.
Chicago
Yoshihara, C., Hamada, K., Koyama, Y."Preparation of a novel adenovirus formulation with artificial envelope of multilayer polymer-coatings: Therapeutic effect on metastatic ovarian cancer". Oncology Reports 23, no. 3 (2010): 733-738. https://doi.org/10.3892/or_00000691