Intratumoral injection of pEGFC1-IGFBP7 inhibits malignant melanoma growth in C57BL/6J mice by inducing apoptosis and down-regulating VEGF expression

  • Authors:
    • Rong-Yi Chen
    • Hong-Xiang Chen
    • Ping Jian
    • Li Xu
    • Juan Li
    • Yi-Ming Fan
    • Ya-Ting Tu
  • View Affiliations

  • Published online on: April 1, 2010     https://doi.org/10.3892/or_00000723
  • Pages: 981-988
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Abstract

Malignant melanoma (MM) is a type of aggressive skin cancer, and the effective therapy for MM is highly desired. Recently, genome-wide RNA interference screening study revealed that loss of expression of insulin-like growth factor binding protein 7 (IGFBP-7) is a critical step in development of MM, and this secreted protein plays a central role in apoptosis of MM. Furthermore, a prostatic carcinoma cell line stably transfected with IGFBP-7 cDNA showed poor tumorigenicity. Thus, we supposed it to be an efficacious agent for inhibiting melanomas. In this study, we constructed pEGFC1-IGFBP7 to try to obtain high expression of IGFPB7 and then we demonstrated that this plasmid inhibited proliferation of B16-F10 melanoma cells efficiently in vitro. Moreover, intratumoral injection of pEGFC1-IGFBP7 inhibited MM growth in C57BL/6J mice. The inhibition of MM growth is due to apoptosis and reduced expression of VEGF induced by pEGFC1-IGFBP7. These results suggest a potential new clinical strategy for MM treatment.

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April 2010
Volume 23 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chen R, Chen H, Jian P, Xu L, Li J, Fan Y and Tu Y: Intratumoral injection of pEGFC1-IGFBP7 inhibits malignant melanoma growth in C57BL/6J mice by inducing apoptosis and down-regulating VEGF expression . Oncol Rep 23: 981-988, 2010
APA
Chen, R., Chen, H., Jian, P., Xu, L., Li, J., Fan, Y., & Tu, Y. (2010). Intratumoral injection of pEGFC1-IGFBP7 inhibits malignant melanoma growth in C57BL/6J mice by inducing apoptosis and down-regulating VEGF expression . Oncology Reports, 23, 981-988. https://doi.org/10.3892/or_00000723
MLA
Chen, R., Chen, H., Jian, P., Xu, L., Li, J., Fan, Y., Tu, Y."Intratumoral injection of pEGFC1-IGFBP7 inhibits malignant melanoma growth in C57BL/6J mice by inducing apoptosis and down-regulating VEGF expression ". Oncology Reports 23.4 (2010): 981-988.
Chicago
Chen, R., Chen, H., Jian, P., Xu, L., Li, J., Fan, Y., Tu, Y."Intratumoral injection of pEGFC1-IGFBP7 inhibits malignant melanoma growth in C57BL/6J mice by inducing apoptosis and down-regulating VEGF expression ". Oncology Reports 23, no. 4 (2010): 981-988. https://doi.org/10.3892/or_00000723