The role of hLHX6-HMR as a methylation biomarker for early diagnosis of cervical cancer

  • Authors:
    • Samil Jung
    • Dongjun Jeong
    • Jinsun Kim
    • Lisha Yi
    • Keunhoe Koo
    • Jaehyouk Lee
    • Soon-Duck Lee
    • Jin-Wha Park
    • Boogi Chang
    • Chang-Hwan Kim
    • Chang-Jin Kim
    • Myeong-Sok Lee
  • View Affiliations

  • Published online on: June 1, 2010     https://doi.org/10.3892/or_00000811
  • Pages: 1675-1682
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Abstract

The homo sapiens LIM homeobox domain LHX6 gene, hLHX6, is a putative transcription regulator with homeo-domain. Multiple cytosine guanine dinucleotides (CpG island) are found in the genomic sequences between exon 4a and exon 5 of the gene encoding hLHX6s (alternative short iso-form of hLHX6 gene). This specific CpG island, hLHX6-HMR, is found frequently hypermethylated in 7 cervical cancer cell lines as shown in MSP, BSP, and COBRA assays. Methylation densities were also investigated with human tissue samples with a distinctive degree of malignant transformation. Our data showed that the hLHX6-HMR was rarely or partly methylated in the normal and CIN I cells, respectively. In contrast, it was frequently hypermethylated in CIN II, CIN III, and invasive carcinoma cells. In summary, this methylation study led to two conclusions. First, hLHX6-HMR hypermethylation is exclusively associated with cervical carcinogenesis. Second, the epigenetic change in hLHX6-HMR seems to start at CIN I, relatively early stage of cervical cancer development. Therefore, hLHX6-HMR can be used as an effective and sensitive methylation biomarker for early diagnosis of cervical cancer.

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June 2010
Volume 23 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Jung S, Jeong D, Kim J, Yi L, Koo K, Lee J, Lee S, Park J, Chang B, Kim C, Kim C, et al: The role of hLHX6-HMR as a methylation biomarker for early diagnosis of cervical cancer . Oncol Rep 23: 1675-1682, 2010.
APA
Jung, S., Jeong, D., Kim, J., Yi, L., Koo, K., Lee, J. ... Lee, M. (2010). The role of hLHX6-HMR as a methylation biomarker for early diagnosis of cervical cancer . Oncology Reports, 23, 1675-1682. https://doi.org/10.3892/or_00000811
MLA
Jung, S., Jeong, D., Kim, J., Yi, L., Koo, K., Lee, J., Lee, S., Park, J., Chang, B., Kim, C., Kim, C., Lee, M."The role of hLHX6-HMR as a methylation biomarker for early diagnosis of cervical cancer ". Oncology Reports 23.6 (2010): 1675-1682.
Chicago
Jung, S., Jeong, D., Kim, J., Yi, L., Koo, K., Lee, J., Lee, S., Park, J., Chang, B., Kim, C., Kim, C., Lee, M."The role of hLHX6-HMR as a methylation biomarker for early diagnosis of cervical cancer ". Oncology Reports 23, no. 6 (2010): 1675-1682. https://doi.org/10.3892/or_00000811