FBXO31 is down-regulated and may function as a tumor suppressor in hepatocellular carcinoma

  • Authors:
    • Hai-Li Huang
    • Wen-Ling Zheng
    • Rui Zhao
    • Bao Zhang
    • Wen-Li Ma
  • View Affiliations

  • Published online on: September 1, 2010     https://doi.org/10.3892/or_00000912
  • Pages: 715-720
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Abstract

The F-box protein family member FBXO31 has rarely been studied in human hepatocellular carcinoma (HCC). This study was designed to investigate the expression profile of FBXO31 in HCC and the possibility that FBXO31 might function as a tumor suppressor in HCC cell lines. We report that FBXO31 is strongly down-regulated in HCC cell lines and specimens. Ectopic expression of FBXO31 inhibited cell proliferation and colony formation in HepG2 and Hep3B cells. The endogenous expression of FBXO31 was fluctuated through cell cycle in the HepG2 cells with maximal expression from late G2 to early G1 phase. Ectopic expression of FBXO31 in HepG2 resulted in the accumulation of cells at the G1 phase of the cell cycle. Possible mechanism might be cyclin D1 degradation mediate by FBXO31 through ubiquitin ligase pathway. Ectopic overexpression of FBXO31 resulted in down-regulation of cyclin D1 which leads to the accumulation of cells at the G1 phase of the cell cycle. Cytoplasmic location of FBXO31 was consistent with cyclin D1 degradation in cytoplasm. Together, our findings suggested that down-regulation of FBXO31 might function as a tumor suppressor in HCC.

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September 2010
Volume 24 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Huang H, Zheng W, Zhao R, Zhang B and Ma W: FBXO31 is down-regulated and may function as a tumor suppressor in hepatocellular carcinoma . Oncol Rep 24: 715-720, 2010
APA
Huang, H., Zheng, W., Zhao, R., Zhang, B., & Ma, W. (2010). FBXO31 is down-regulated and may function as a tumor suppressor in hepatocellular carcinoma . Oncology Reports, 24, 715-720. https://doi.org/10.3892/or_00000912
MLA
Huang, H., Zheng, W., Zhao, R., Zhang, B., Ma, W."FBXO31 is down-regulated and may function as a tumor suppressor in hepatocellular carcinoma ". Oncology Reports 24.3 (2010): 715-720.
Chicago
Huang, H., Zheng, W., Zhao, R., Zhang, B., Ma, W."FBXO31 is down-regulated and may function as a tumor suppressor in hepatocellular carcinoma ". Oncology Reports 24, no. 3 (2010): 715-720. https://doi.org/10.3892/or_00000912