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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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October 2010 Volume 24 Issue 4

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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October 2010 Volume 24 Issue 4

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Article

β-catenin siRNA regulation of apoptosis- and angiogenesis-related gene expression in hepatocellular carcinoma cells: potential uses for gene therapy

  • Authors:
    • Xin-Hong Wang
    • Xun Sun
    • Xiang-Wei Meng
    • Zhi-Wu Lv
    • Ya-Ju Du
    • Yan Zhu
    • Jing Chen
    • De-Xia Kong
    • Shi-Zhu Jin

  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, the First Hospital of Jilin University, Changchun, Jilin, P.R. China
  • Pages: 1093-1099
    |
    Published online on: October 1, 2010
       https://doi.org/10.3892/or_00000960
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Abstract

The molecular mechanism responsible for hepatocellular carcinoma (HCC) development remains to be defined although a number of gene pathways have been shown to play an active role, such as Wnt/β-catenin signaling. In this study, β-catenin small interfering RNA (siRNA) was designed, synthesized, and transfected into HCC HepG2 cells. RT-PCR and western blot assays were performed to detect expression of altered genes and proteins, and the MTT assay was used to detect cell viability. Our data showed that β-catenin mRNA and protein expression levels were effectively knocked down by β-catenin siRNA and subsequently, tumor cell proliferation was significantly suppressed. Flow cytometry assay showed that tumor cells were arrested at the G0/G1 phase of the cell cycles. Molecularly, expression of Smad3, p-caspase-3, and Grp78 protein were upregulated after 72 h of β-catenin siRNA transfection, whereas expression of TERT, caspase-3, XIAP, MMP-2, MMP-9, VEGF-A, VEGF-c, and bFGF protein were reduced. However, there was no change between the expression of STAT3 and the HSP27 protein following transfection. The results from the current study demonstrated the importance of the Wnt/β-catenin signaling pathway in regulation of gene expression in HCC. Further studies are required to investigate the role of this pathway in HCC development and targeting of this pathway to control HCC.

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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Sun X, Meng X, Lv Z, Du Y, Zhu Y, Chen J, Kong D and Jin S: β-catenin siRNA regulation of apoptosis- and angiogenesis-related gene expression in hepatocellular carcinoma cells: potential uses for gene therapy . Oncol Rep 24: 1093-1099, 2010.
APA
Wang, X., Sun, X., Meng, X., Lv, Z., Du, Y., Zhu, Y. ... Jin, S. (2010). β-catenin siRNA regulation of apoptosis- and angiogenesis-related gene expression in hepatocellular carcinoma cells: potential uses for gene therapy . Oncology Reports, 24, 1093-1099. https://doi.org/10.3892/or_00000960
MLA
Wang, X., Sun, X., Meng, X., Lv, Z., Du, Y., Zhu, Y., Chen, J., Kong, D., Jin, S."β-catenin siRNA regulation of apoptosis- and angiogenesis-related gene expression in hepatocellular carcinoma cells: potential uses for gene therapy ". Oncology Reports 24.4 (2010): 1093-1099.
Chicago
Wang, X., Sun, X., Meng, X., Lv, Z., Du, Y., Zhu, Y., Chen, J., Kong, D., Jin, S."β-catenin siRNA regulation of apoptosis- and angiogenesis-related gene expression in hepatocellular carcinoma cells: potential uses for gene therapy ". Oncology Reports 24, no. 4 (2010): 1093-1099. https://doi.org/10.3892/or_00000960
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Sun X, Meng X, Lv Z, Du Y, Zhu Y, Chen J, Kong D and Jin S: β-catenin siRNA regulation of apoptosis- and angiogenesis-related gene expression in hepatocellular carcinoma cells: potential uses for gene therapy . Oncol Rep 24: 1093-1099, 2010.
APA
Wang, X., Sun, X., Meng, X., Lv, Z., Du, Y., Zhu, Y. ... Jin, S. (2010). β-catenin siRNA regulation of apoptosis- and angiogenesis-related gene expression in hepatocellular carcinoma cells: potential uses for gene therapy . Oncology Reports, 24, 1093-1099. https://doi.org/10.3892/or_00000960
MLA
Wang, X., Sun, X., Meng, X., Lv, Z., Du, Y., Zhu, Y., Chen, J., Kong, D., Jin, S."β-catenin siRNA regulation of apoptosis- and angiogenesis-related gene expression in hepatocellular carcinoma cells: potential uses for gene therapy ". Oncology Reports 24.4 (2010): 1093-1099.
Chicago
Wang, X., Sun, X., Meng, X., Lv, Z., Du, Y., Zhu, Y., Chen, J., Kong, D., Jin, S."β-catenin siRNA regulation of apoptosis- and angiogenesis-related gene expression in hepatocellular carcinoma cells: potential uses for gene therapy ". Oncology Reports 24, no. 4 (2010): 1093-1099. https://doi.org/10.3892/or_00000960
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