Sodium valproate, a histone deacetylase inhibitor, augments the expression of cell-surface NKG2D ligands, MICA/B, without increasing their soluble forms to enhance susceptibility of human osteosarcoma cells to NK cell-mediated cytotoxicity

  • Authors:
    • Koji Yamanegi
    • Junko Yamane
    • Kenta Kobayashi
    • Nahoko Kato-Kogoe
    • Hideki Ohyama
    • Keiji Nakasho
    • Naoko Yamada
    • Masaki Hata
    • Toshihiro Nishioka
    • Satoru Fukunaga
    • Hiroyuki Futani
    • Haruki Okamura
    • Nobuyuki Terada
  • View Affiliations

  • Published online on: December 1, 2010     https://doi.org/10.3892/or_00001026
  • Pages: 1621-1627
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Abstract

MHC class I-related chain molecules A and B (MICA and B) expressed on the cell-surface of tumor cells are ligands for an activating receptor, NKG2D, expressed on natural killer (NK) cells and stimulate the NK cell-mediated cytotoxicity. On the other hand, the soluble form of MICA and B produced by proteolytic cleavage of cell-surface MIC interferes with NK cell-mediated cytotoxicity. We investigated effect of sodium valproate (VPA), a histone deacetylase inhibitor, on the production of cell-surface and soluble MICA and B and NK cell-mediated cytotoxicity in four human osteosarcoma cells. VPA at 0.5 and 1.0 mM induced acetylation of histones bound to MICA and B gene promoters, increased cell-surface but not soluble MICA and B, and augmented the susceptibility of osteosarcoma cells to NK cell-mediated cytotoxicity. The present results indicate that VPA sensitizes human osteosarcoma cells to cytotoxicity of NK cells.

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December 2010
Volume 24 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yamanegi K, Yamane J, Kobayashi K, Kato-Kogoe N, Ohyama H, Nakasho K, Yamada N, Hata M, Nishioka T, Fukunaga S, Fukunaga S, et al: Sodium valproate, a histone deacetylase inhibitor, augments the expression of cell-surface NKG2D ligands, MICA/B, without increasing their soluble forms to enhance susceptibility of human osteosarcoma cells to NK cell-mediated cytotoxicity . Oncol Rep 24: 1621-1627, 2010
APA
Yamanegi, K., Yamane, J., Kobayashi, K., Kato-Kogoe, N., Ohyama, H., Nakasho, K. ... Terada, N. (2010). Sodium valproate, a histone deacetylase inhibitor, augments the expression of cell-surface NKG2D ligands, MICA/B, without increasing their soluble forms to enhance susceptibility of human osteosarcoma cells to NK cell-mediated cytotoxicity . Oncology Reports, 24, 1621-1627. https://doi.org/10.3892/or_00001026
MLA
Yamanegi, K., Yamane, J., Kobayashi, K., Kato-Kogoe, N., Ohyama, H., Nakasho, K., Yamada, N., Hata, M., Nishioka, T., Fukunaga, S., Futani, H., Okamura, H., Terada, N."Sodium valproate, a histone deacetylase inhibitor, augments the expression of cell-surface NKG2D ligands, MICA/B, without increasing their soluble forms to enhance susceptibility of human osteosarcoma cells to NK cell-mediated cytotoxicity ". Oncology Reports 24.6 (2010): 1621-1627.
Chicago
Yamanegi, K., Yamane, J., Kobayashi, K., Kato-Kogoe, N., Ohyama, H., Nakasho, K., Yamada, N., Hata, M., Nishioka, T., Fukunaga, S., Futani, H., Okamura, H., Terada, N."Sodium valproate, a histone deacetylase inhibitor, augments the expression of cell-surface NKG2D ligands, MICA/B, without increasing their soluble forms to enhance susceptibility of human osteosarcoma cells to NK cell-mediated cytotoxicity ". Oncology Reports 24, no. 6 (2010): 1621-1627. https://doi.org/10.3892/or_00001026