mda-7/IL-24 induces apoptosis in human GBC-SD gallbladder carcinoma cells via mitochondrial apoptotic pathway

  • Authors:
    • Jianguang Jia
    • Songgang Li
    • Wei Gong
    • Jing Ding
    • Cuifu Fang
    • Zhiwei Quan
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  • Published online on: January 1, 2011     https://doi.org/10.3892/or_00001061
  • Pages: 195-201
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Abstract

mda-7/IL-24 has tumor-suppressor activity in a broad spectrum of human cancer cells. However, the therapeutic effect of the recombinant human IL-24 protein on human gallbladder carcinoma has rarely been explored. In this study, we used a human gallbladder carcinoma cell line (GBC-SD) to explore the effect of adenovirus-mediated IL-24 (Ad-IL24) gene therapy on GBC-SD cells. We show that Ad-IL24 treatment of GBC-SD cells in vitro conspicuously induced apoptosis of GBC-SD cells. We also demonstrate that the in vivo treatment of GBC tumor-bearing athymic nude mice intratumorally injected with Ad-IL24 significantly suppressed GBC growth. To further explore the mechanism that mda-7/IL-24 utilized in tumor cell apoptosis, we examined molecules and pathways involved in apoptotic regulation and found that Ad-IL24 induced the down-regulation of anti-apoptotic gene Bcl-2 and the release of cytochrome c, which subsequently activated caspase-9, caspase-3 and PARP to induce apoptosis. In summary, adenovirus (AdV)-mediated IL-24 overexpression exerted potent antitumor activity via stimulating mitochondrial apoptotic pathway in GBC-SD. Therefore, mda-7/IL-24 has the potential to serve as a tool for targeted gene therapy in the treatment of gallbladder cancer.

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January 2011
Volume 25 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Jia J, Li S, Gong W, Ding J, Fang C and Quan Z: mda-7/IL-24 induces apoptosis in human GBC-SD gallbladder carcinoma cells via mitochondrial apoptotic pathway . Oncol Rep 25: 195-201, 2011.
APA
Jia, J., Li, S., Gong, W., Ding, J., Fang, C., & Quan, Z. (2011). mda-7/IL-24 induces apoptosis in human GBC-SD gallbladder carcinoma cells via mitochondrial apoptotic pathway . Oncology Reports, 25, 195-201. https://doi.org/10.3892/or_00001061
MLA
Jia, J., Li, S., Gong, W., Ding, J., Fang, C., Quan, Z."mda-7/IL-24 induces apoptosis in human GBC-SD gallbladder carcinoma cells via mitochondrial apoptotic pathway ". Oncology Reports 25.1 (2011): 195-201.
Chicago
Jia, J., Li, S., Gong, W., Ding, J., Fang, C., Quan, Z."mda-7/IL-24 induces apoptosis in human GBC-SD gallbladder carcinoma cells via mitochondrial apoptotic pathway ". Oncology Reports 25, no. 1 (2011): 195-201. https://doi.org/10.3892/or_00001061