Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
World Academy of Sciences Journal
Join Editorial Board Propose a Special Issue
Print ISSN: 2632-2900 Online ISSN: 2632-2919
Journal Cover
March-April 2019 Volume 1 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-April 2019 Volume 1 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Effects of irradiated cell conditioned medium on the response of human lung cancer cells to anticancer treatment in vitro

  • Authors:
    • Hironori Yoshino
    • Mikoto Nawamaki
    • Kanna Murakami
    • Ikuo Kashiwakura
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Science, Hirosaki University Graduate School of Health Sciences, Hirosaki, Aomori 036‑8564, Japan, Department of Radiological Technology, Hirosaki University School of Health Sciences, Hirosaki, Aomori 036‑8564, Japan
  • Pages: 92-97
    |
    Published online on: April 24, 2019
       https://doi.org/10.3892/wasj.2019.11
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Ionizing radiation induces a cellular response not only in the irradiated cells, but also in the neighboring non‑irradiated cells, and this effect is known as the radiation‑induced bystander effect. The irradiated cells transmit signals to the non‑irradiated cells via gap junctions or soluble factors, exerting biological effects on the neighboring non‑irradiated cells. In this study, we investigated the effects of irradiated cell conditioned medium (ICCM) on the response of human lung cancer cells (A549 and H1299) to anticancer treatment. First, we analyzed the effects of ICCM on the induction of apoptosis by anticancer treatment (ionizing radiation or gefitinib). Human lung cancer cells were cultured with ICCM from non‑irradiated and 8 Gy‑irradiated cells and, subsequently, exposed to 8 Gy X‑ray. In the A549 cells, the proportion of Annexin V+ apoptotic cells was significantly lower in the cells treated with ICCM from 8 Gy‑irradiated cells when compared with that in the cells treated with ICCM from non‑irradiated cells (P<0.05), whereas this effect was not observed in the H1299 cells. Furthermore, no significant difference in the proportion of Annexin V+ cells was noted following treatment with gefitinib, an inhibitor of the epidermal growth factor receptor tyrosine kinase, between the cells treated with ICCM from non‑irradiated cells and those treated with ICCM from the 8 Gy‑irradiated cells. We then examined the effects of ICCM on the migration of A549 cells by scratch assay. ICCM from the 8 Gy‑irradiated cells enhanced the migration of the 8 Gy‑irradiated cells, but not that of the non‑irradiated cells. Taken together, these results suggest that cancer cells treated with ICCM exhibit resistance to ionizing radiation in terms of apoptosis and cellular migration, although this phenomenon depends on the cell type.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Tomita M and Maeda M: Mechanisms and biological importance of photon-induced bystander responses: Do they have an impact on low-dose radiation responses. J Radiat Res (Tokyo). 56:205–219. 2015.PubMed/NCBI View Article : Google Scholar

2 

Hamada N, Maeda M, Otsuka K and Tomita M: Signaling pathways underpinning the manifestations of ionizing radiation-induced bystander effects. Curr Mol Pharmacol. 4:79–95. 2011.PubMed/NCBI View Article : Google Scholar

3 

Matsumoto H, Tomita M, Otsuka K, Hatashita M and Hamada N: Nitric oxide is a key molecule serving as a bridge between radiation-induced bystander and adaptive responses. Curr Mol Pharmacol. 4:126–134. 2011.PubMed/NCBI View Article : Google Scholar

4 

Kadhim MA and Hill MA: Non-targeted effects of radiation exposure: Recent advances and implications. Radiat Prot Dosimetry. 166:118–124. 2015.PubMed/NCBI View Article : Google Scholar

5 

Grass GD, Krishna N and Kim S: The immune mechanisms of abscopal effect in radiation therapy. Curr Probl Cancer. 40:10–24. 2016.PubMed/NCBI View Article : Google Scholar

6 

Siva S, MacManus MP, Martin RF and Martin OA: Abscopal effects of radiation therapy: A clinical review for the radiobiologist. Cancer Lett. 356:82–90. 2015.PubMed/NCBI View Article : Google Scholar

7 

Levy A, Chargari C, Marabelle A, Perfettini JL, Magné N and Deutsch E: Can immunostimulatory agents enhance the abscopal effect of radiotherapy? Eur J Cancer. 62:36–45. 2016.PubMed/NCBI View Article : Google Scholar

8 

Demaria S, Ng B, Devitt ML, Babb JS, Kawashima N, Liebes L and Formenti SC: Ionizing radiation inhibition of distant untreated tumors (abscopal effect) is immune mediated. Int J Radiat Oncol Biol Phys. 58:862–870. 2004.PubMed/NCBI View Article : Google Scholar

9 

Yang S, Xu J, Shao W, Geng C, Li J, Guo F, Miao H, Shen W, Ye T, Liu Y, et al: Radiation-induced bystander effects in A549 cells exposed to 6 MV X-rays. Cell Biochem Biophys. 72:877–882. 2015.PubMed/NCBI View Article : Google Scholar

10 

Jiang Y, Chen X, Tian W, Yin X, Wang J and Yang H: The role of TGF-β1-miR-21-ROS pathway in bystander responses induced by irradiated non-small-cell lung cancer cells. Br J Cancer. 111:772–780. 2014.PubMed/NCBI View Article : Google Scholar

11 

Yoshino H, Murakami K, Nawamaki M and Kashiwakura I: Effects of Nrf2 knockdown on the properties of irradiated cell conditioned medium from A549 human lung cancer cells. Biomed Rep. 8:461–465. 2018.PubMed/NCBI View Article : Google Scholar

12 

Yoshino H, Konno H, Ogura K, Sato Y and Kashiwakura I: Relationship between the regulation of caspase-8-mediated apoptosis and radioresistance in human THP-1-derived macrophages. Int J Mol Sci. 19(E3154)2018.PubMed/NCBI View Article : Google Scholar

13 

Morgan WF and Sowa MB: Non-targeted effects induced by ionizing radiation: Mechanisms and potential impact on radiation induced health effects. Cancer Lett. 356:17–21. 2015.PubMed/NCBI View Article : Google Scholar

14 

Kucharczuk CR, Ganetsky A and Vozniak JM: Drug-drug interactions, safety, and pharmacokinetics of EGFR tyrosine kinase inhibitors for the treatment of non-small cell lung cancer. J Adv Pract Oncol. 9:189–200. 2018.PubMed/NCBI

15 

Iyer R and Lehnert BE: Low dose, low-LET ionizing radiation-induced radioadaptation and associated early responses in unirradiated cells. Mutat Res. 503:1–9. 2002.PubMed/NCBI View Article : Google Scholar

16 

Pasi F, Facoetti A and Nano R: IL-8 and IL-6 bystander signalling in human glioblastoma cells exposed to gamma radiation. Anticancer Res. 30:2769–2772. 2010.PubMed/NCBI

17 

Tamari Y, Kashino G and Mori H: Acquisition of radioresistance by IL-6 treatment is caused by suppression of oxidative stress derived from mitochondria after γ-irradiation. J Radiat Res (Tokyo). 58:412–420. 2017.PubMed/NCBI View Article : Google Scholar

18 

Takahashi A, Matsumoto H and Ohnishi T: Hdm2 and nitric oxide radicals contribute to the p53-dependent radioadaptive response. Int J Radiat Oncol Biol Phys. 71:550–558. 2008.PubMed/NCBI View Article : Google Scholar

19 

Yonezawa M: Induction of radio-resistance by low dose X-irradiation. Yakugaku Zasshi. 126:833–840. 2006.(In Japanese). PubMed/NCBI View Article : Google Scholar

20 

Lakin ND and Jackson SP: Regulation of p53 in response to DNA damage. Oncogene. 18:7644–7655. 1999.PubMed/NCBI View Article : Google Scholar

21 

Kim JJ and Tannock IF: Repopulation of cancer cells during therapy: An important cause of treatment failure. Nat Rev Cancer. 5:516–525. 2005.PubMed/NCBI View Article : Google Scholar

22 

Rho JK, Choi YJ, Ryoo BY, Na II, Yang SH, Kim CH and Lee JC: p53 enhances gefitinib-induced growth inhibition and apoptosis by regulation of Fas in non-small cell lung cancer. Cancer Res. 67:1163–1169. 2007.PubMed/NCBI View Article : Google Scholar

23 

Klammer H, Mladenov E, Li F and Iliakis G: Bystander effects as manifestation of intercellular communication of DNA damage and of the cellular oxidative status. Cancer Lett. 356:58–71. 2015.PubMed/NCBI View Article : Google Scholar

24 

Kuwahara Y, Roudkenar MH, Suzuki M, Urushihara Y and Fukumoto M, Saito Y and Fukumoto M: The involvement of mitochondrial membrane potential in cross-resistance between radiation and docetaxel. Int J Radiat Oncol Biol Phys. 96:556–565. 2016.PubMed/NCBI View Article : Google Scholar

25 

Akino Y, Teshima T, Kihara A, Kodera-Suzumoto Y, Inaoka M, Higashiyama S, Furusawa Y and Matsuura N: Carbon-ion beam irradiation effectively suppresses migration and invasion of human non-small-cell lung cancer cells. Int J Radiat Oncol Biol Phys. 75:475–481. 2009.PubMed/NCBI View Article : Google Scholar

26 

Zhou YC, Liu JY, Li J, Zhang J, Xu YQ, Zhang HW, Qiu LB, Ding GR, Su XM, Mei Shi and Guo GZ: Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-beta-mediated epithelial-mesenchymal transition. Int J Radiat Oncol Biol Phys. 81:1530–1537. 2011.PubMed/NCBI View Article : Google Scholar

27 

Gow MD, Seymour CB, Ryan LA and Mothersill CE: Induction of bystander response in human glioma cells using high-energy electrons: A role for TGF-beta1. Radiat Res. 173:769–778. 2010.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
Copy and paste a formatted citation
Spandidos Publications style
Yoshino H, Nawamaki M, Murakami K and Kashiwakura I: Effects of irradiated cell conditioned medium on the response of human lung cancer cells to anticancer treatment in vitro . World Acad Sci J 1: 92-97, 2019.
APA
Yoshino, H., Nawamaki, M., Murakami, K., & Kashiwakura, I. (2019). Effects of irradiated cell conditioned medium on the response of human lung cancer cells to anticancer treatment in vitro . World Academy of Sciences Journal, 1, 92-97. https://doi.org/10.3892/wasj.2019.11
MLA
Yoshino, H., Nawamaki, M., Murakami, K., Kashiwakura, I."Effects of irradiated cell conditioned medium on the response of human lung cancer cells to anticancer treatment in vitro ". World Academy of Sciences Journal 1.2 (2019): 92-97.
Chicago
Yoshino, H., Nawamaki, M., Murakami, K., Kashiwakura, I."Effects of irradiated cell conditioned medium on the response of human lung cancer cells to anticancer treatment in vitro ". World Academy of Sciences Journal 1, no. 2 (2019): 92-97. https://doi.org/10.3892/wasj.2019.11
Copy and paste a formatted citation
x
Spandidos Publications style
Yoshino H, Nawamaki M, Murakami K and Kashiwakura I: Effects of irradiated cell conditioned medium on the response of human lung cancer cells to anticancer treatment in vitro . World Acad Sci J 1: 92-97, 2019.
APA
Yoshino, H., Nawamaki, M., Murakami, K., & Kashiwakura, I. (2019). Effects of irradiated cell conditioned medium on the response of human lung cancer cells to anticancer treatment in vitro . World Academy of Sciences Journal, 1, 92-97. https://doi.org/10.3892/wasj.2019.11
MLA
Yoshino, H., Nawamaki, M., Murakami, K., Kashiwakura, I."Effects of irradiated cell conditioned medium on the response of human lung cancer cells to anticancer treatment in vitro ". World Academy of Sciences Journal 1.2 (2019): 92-97.
Chicago
Yoshino, H., Nawamaki, M., Murakami, K., Kashiwakura, I."Effects of irradiated cell conditioned medium on the response of human lung cancer cells to anticancer treatment in vitro ". World Academy of Sciences Journal 1, no. 2 (2019): 92-97. https://doi.org/10.3892/wasj.2019.11
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team