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Article Open Access

Effect of ultrasound exposure on the Triticum aestivum root elongation test

  • Authors:
    • Iulia Marina Potolea
    • Oana Marina Pîrvu
    • Cristiana Elena Spinu
    • Dragoş Paul Mihai
    • Octavian Tudorel Olaru
    • George Mihai Niţulescu
    • Aristidis Tsatsakis
    • Demetrios A. Spandidos
    • Georgiana Niţulescu
  • View Affiliations / Copyright

    Affiliations: Faculty of Pharmacy, ‘Carol Davila’ University of Medicine and Pharmacy, 020956 Bucharest, Romania, Department of Forensic Sciences and Toxicology, School of Medicine, University of Crete, 71003 Heraklion, Greece, Laboratory of Clinical Virology, School of Medicine, University of Crete, 71003 Heraklion, Greece
    Copyright: © Potolea et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 290-295
    |
    Published online on: November 18, 2019
       https://doi.org/10.3892/wasj.2019.27
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Abstract

The Triticum aestivum root elongation test can be used as a simple and effective method for the identification of novel potential anti‑proliferative agents. The employment of this method is based on the fact that a significant number of oncogenic signal transduction pathways are highly conserved in living organisms. Moreover, the use of phytobiological assays has several advantages, such as rapid outcomes, simple procedure and reduced costs. Previous studies have demonstrated that ultrasound exposure can have lethal effects on small organisms, medium and high frequency exposure, leading to cell destruction. In the present study, the effects of ultrasound exposure on the radicular length of Triticum caryopses were analyzed as a potential tool for further experimentation using ultrasounds as a stress model in phytobiological tests. The influence of the exposure time was determined at different intervals and was proven to exert significant effects. Short intervals of <5 min produced no statistically significant effects on root elongation, and the maximum of inhibition was achieved following 30 min of exposure. The experimental results highlighted that the inhibitory effect of ultrasound irradiation was lower following 15 min of exposure than after a shorter interval of 10 min, emphasizing a complex, non‑linear biological response. On the whole, the findings of this study indicated a complex mechanism for the inhibition of the radicular growth by ultrasound exposure. The ultrasound exposure of Triticum seeds may be used as a tool to either assess the effects of toxic agents following an increase in cell permeability, or to examine the synergistic action between ultrasounds and exposure to chemical mixtures that can promote physiological disruptions.
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Spandidos Publications style
Potolea IM, Pîrvu OM, Spinu CE, Mihai DP, Olaru OT, Niţulescu GM, Tsatsakis A, Spandidos DA and Niţulescu G: Effect of ultrasound exposure on the Triticum aestivum root elongation test. World Acad Sci J 1: 290-295, 2019.
APA
Potolea, I.M., Pîrvu, O.M., Spinu, C.E., Mihai, D.P., Olaru, O.T., Niţulescu, G.M. ... Niţulescu, G. (2019). Effect of ultrasound exposure on the Triticum aestivum root elongation test. World Academy of Sciences Journal, 1, 290-295. https://doi.org/10.3892/wasj.2019.27
MLA
Potolea, I. M., Pîrvu, O. M., Spinu, C. E., Mihai, D. P., Olaru, O. T., Niţulescu, G. M., Tsatsakis, A., Spandidos, D. A., Niţulescu, G."Effect of ultrasound exposure on the Triticum aestivum root elongation test". World Academy of Sciences Journal 1.6 (2019): 290-295.
Chicago
Potolea, I. M., Pîrvu, O. M., Spinu, C. E., Mihai, D. P., Olaru, O. T., Niţulescu, G. M., Tsatsakis, A., Spandidos, D. A., Niţulescu, G."Effect of ultrasound exposure on the Triticum aestivum root elongation test". World Academy of Sciences Journal 1, no. 6 (2019): 290-295. https://doi.org/10.3892/wasj.2019.27
Copy and paste a formatted citation
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Spandidos Publications style
Potolea IM, Pîrvu OM, Spinu CE, Mihai DP, Olaru OT, Niţulescu GM, Tsatsakis A, Spandidos DA and Niţulescu G: Effect of ultrasound exposure on the Triticum aestivum root elongation test. World Acad Sci J 1: 290-295, 2019.
APA
Potolea, I.M., Pîrvu, O.M., Spinu, C.E., Mihai, D.P., Olaru, O.T., Niţulescu, G.M. ... Niţulescu, G. (2019). Effect of ultrasound exposure on the Triticum aestivum root elongation test. World Academy of Sciences Journal, 1, 290-295. https://doi.org/10.3892/wasj.2019.27
MLA
Potolea, I. M., Pîrvu, O. M., Spinu, C. E., Mihai, D. P., Olaru, O. T., Niţulescu, G. M., Tsatsakis, A., Spandidos, D. A., Niţulescu, G."Effect of ultrasound exposure on the Triticum aestivum root elongation test". World Academy of Sciences Journal 1.6 (2019): 290-295.
Chicago
Potolea, I. M., Pîrvu, O. M., Spinu, C. E., Mihai, D. P., Olaru, O. T., Niţulescu, G. M., Tsatsakis, A., Spandidos, D. A., Niţulescu, G."Effect of ultrasound exposure on the Triticum aestivum root elongation test". World Academy of Sciences Journal 1, no. 6 (2019): 290-295. https://doi.org/10.3892/wasj.2019.27
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