Effect of ultrasound exposure on the Triticum aestivum root elongation test

Potolea,Iulia  Marina; Pîrvu,Oana   Marina; Spinu,Cristiana   Elena; Mihai,Dragoş   Paul; Olaru,Octavian   Tudorel; Niţulescu,George   Mihai; Tsatsakis,Aristidis; Spandidos,Demetrios   A.; Niţulescu,Georgiana

doi:10.3892/wasj.2019.27

Effect of ultrasound exposure on the Triticum aestivum root elongation test

Authors:
- Iulia Marina Potolea
- Oana Marina Pîrvu
- Cristiana Elena Spinu
- Dragoş Paul Mihai
- Octavian Tudorel Olaru
- George Mihai Niţulescu
- Aristidis Tsatsakis
- Demetrios A. Spandidos
- Georgiana Niţulescu
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Affiliations: Faculty of Pharmacy, ‘Carol Davila’ University of Medicine and Pharmacy, 020956 Bucharest, Romania, Department of Forensic Sciences and Toxicology, School of Medicine, University of Crete, 71003 Heraklion, Greece, Laboratory of Clinical Virology, School of Medicine, University of Crete, 71003 Heraklion, Greece
Published online on: November 18, 2019 https://doi.org/10.3892/wasj.2019.27
Pages: 290-295
Copyright: © Potolea et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The Triticum aestivum root elongation test can be used as a simple and effective method for the identification of novel potential anti‑proliferative agents. The employment of this method is based on the fact that a significant number of oncogenic signal transduction pathways are highly conserved in living organisms. Moreover, the use of phytobiological assays has several advantages, such as rapid outcomes, simple procedure and reduced costs. Previous studies have demonstrated that ultrasound exposure can have lethal effects on small organisms, medium and high frequency exposure, leading to cell destruction. In the present study, the effects of ultrasound exposure on the radicular length of Triticum caryopses were analyzed as a potential tool for further experimentation using ultrasounds as a stress model in phytobiological tests. The influence of the exposure time was determined at different intervals and was proven to exert significant effects. Short intervals of <5 min produced no statistically significant effects on root elongation, and the maximum of inhibition was achieved following 30 min of exposure. The experimental results highlighted that the inhibitory effect of ultrasound irradiation was lower following 15 min of exposure than after a shorter interval of 10 min, emphasizing a complex, non‑linear biological response. On the whole, the findings of this study indicated a complex mechanism for the inhibition of the radicular growth by ultrasound exposure. The ultrasound exposure of Triticum seeds may be used as a tool to either assess the effects of toxic agents following an increase in cell permeability, or to examine the synergistic action between ultrasounds and exposure to chemical mixtures that can promote physiological disruptions.

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1	Nitulescu GM, Draghici C and Olaru OT: New potential antitumor pyrazole derivatives: Synthesis and cytotoxic evaluation. Int J Mol Sci. 14:21805–21818. 2013.PubMed/NCBI View Article : Google Scholar
2	Olaru OT, Zanfirescu A, Nitulescu GM, Nitulescu G, Dinu-Pirvu CE, Anuta V, Tsatsakis A, Spandidos DA, Margina D and Seremet OC: Predictive power of the Triticum root elongation test for the assessment of novel anti proliferative therapies. Int J Mol Med. 44:16–24. 2019.PubMed/NCBI View Article : Google Scholar
3	Margină D, Olaru OT, Ilie M, Grădinaru D, GuȚu C, Voicu S, Dinischiotu A, Spandidos DA and Tsatsakis AM: Assessment of the potential health benefits of certain total extracts from Vitis vinifera, Aesculus hyppocastanum and Curcuma longa. Exp Ther Med. 10:1681–1688. 2015.PubMed/NCBI View Article : Google Scholar
4	Guzman-Rangel G, Montalvo D and Smolders E: Pronounced Antagonism of Zinc and Arsenate on Toxicity to Barley Root Elongation in Soil. Environments. 5(83)2018. View Article : Google Scholar
5	Zhu G, Jiang B, Yang G, Li J and Ma Y: Toxicity thresholds based on EDTA extractable nickel and barley root elongation in Chinese soils. Int J Environ Res Public Health. 15(15)2018.PubMed/NCBI View Article : Google Scholar
6	Jaskowiak J, Kwasniewska J, Milewska-Hendel A, Kurczynska EU, Szurman-Zubrzycka M and Szarejko I: Aluminum Alters the Histology and Pectin Cell Wall Composition of Barley Roots. Int J Mol Sci. 20(20)2019.PubMed/NCBI View Article : Google Scholar
7	Suslick KS, Hammerton DA and Cline RE: Sonochemical hot spot. J Am Chem Soc. 108:5641–5642. 1986. View Article : Google Scholar
8	Wells PN: The effect of ultrasonic irradiation on the survival of Daphnia magna. J Exp Biol. 49:61–70. 1968.PubMed/NCBI
9	Lürling M and Tolman Y: Effects of Commercially Available Ultrasound on the Zooplankton Grazer Daphnia and Consequent Water Greening in Laboratory Experiments. Water. 6:3247–3263. 2014. View Article : Google Scholar
10	Wang Q, Chen G, Yersaiyiti H, Liu Y, Cui J, Wu C, Zhang Y and He X: Modeling analysis on germination and seedling growth using ultrasound seed pretreatment in switchgrass. PLoS One. 7(e47204)2012.PubMed/NCBI View Article : Google Scholar
11	Karabascheff N, Metev V and Kutov T: On the effect of ultrasonic waves on the germination of corn. C R Acad Bulg Sci. 19:305–312. 1966.(In German). PubMed/NCBI
12	Patero T and Augusto PED: Ultrasound (US) enhances the hydration of sorghum (Sorghum bicolor) grains. Ultrason Sonochem. 23:11–15. 2015.PubMed/NCBI View Article : Google Scholar
13	Ghafoor M, Misra NN, Mahadevan K and Tiwari BK: Ultrasound assisted hydration of navy beans (Phaseolus vulgaris). Ultrason Sonochem. 21:409–414. 2014.PubMed/NCBI View Article : Google Scholar
14	Gîrd CE, Costea T, Duţu LE, Popescu ML and Nencu I: Phytochemical and phytobiological research regarding roots of Heracleum Sphondylium L. and H. Palmatum Baumg. Trends Toxicol Relat Sci. 1:84–93. 2017.
15	Goussous SJ, Samarah NH, Alqudah AM and Othman MO: Enhancing seed germination of four crop species using an ultrasonic technique. Exp Agric. 46:231–242. 2010. View Article : Google Scholar
16	Shin YK, Baque MA, Lee EJ and Paek KY: Effects of activated charcoal, plant growth regulators and ultrasonic pre-treatments on in vitro germination and protocorm formation of Calanthe hybrids. Aust J Crop Sci. 5:582–588. 2011.
17	Wu M, Wang X, Jia Z, De Schamphelaere K, Ji D, Li X and Chen X: Modeling acute toxicity of metal mixtures to wheat (Triticum aestivum L.) using the biotic ligand model-based toxic units method. Sci Rep. 7(9443)2017.PubMed/NCBI View Article : Google Scholar
18	Sadeghianfar P, Nazari M and Backes G: Exposure to Ultraviolet (UV-C) Radiation Increases Germination Rate of Maize (Zea maize L.) and Sugar Beet (Beta vulgaris) Seeds. Plants. 8(49)2019.PubMed/NCBI View Article : Google Scholar
19	Izadifar Z, Babyn P and Chapman D: Mechanical and Biological Effects of Ultrasound: A Review of Present Knowledge. Ultrasound Med Biol. 43:1085–1104. 2017.PubMed/NCBI View Article : Google Scholar
20	Tsatsakis AM, Docea AO and Tsitsimpikou C: New challenges in risk assessment of chemicals when simulating real exposure scenarios; simultaneous multi-chemicals' low dose exposure. Food Chem Toxicol. 96:174–176. 2016.PubMed/NCBI View Article : Google Scholar
21	Margina D, Nițulescu GM, Ungurianu A, Mesnage R, Goumenou M, Sarigiannis DA, Aschner M, Spandidos DA, Renieri EA, Hernández AF, et al: Overview of the effects of chemical mixtures with endocrine disrupting activity in the context of real life risk simulation (RLRS): An integrative approach (Review). World Acad Sci J. 1:157–164. 2019. View Article : Google Scholar
22	Tsatsakis AM, Kouretas D, Tzatzarakis MN, Stivaktakis P, Tsarouhas K, Golokhvast KS, Rakitskii VN, Tutelyan VA, Hernandez AF, Rezaee R, et al: Simulating real-life exposures to uncover possible risks to human health: A proposed consensus for a novel methodological approach. Hum Exp Toxicol. 36:554–564. 2017.PubMed/NCBI View Article : Google Scholar
23	Fountoucidou P, Veskoukis AS, Kerasioti E, Docea AO, Taitzoglou IA, Liesivuori J, Tsatsakis A and Kouretas D: A mixture of routinely encountered xenobiotics induces both redox adaptations and perturbations in blood and tissues of rats after a long-term low-dose exposure regimen: The time and dose issue. Toxicol Lett. 317:24–44. 2019.PubMed/NCBI View Article : Google Scholar
24	Tsatsakis A, Docea AO, Constantin C, Calina D, Zlatian O, Nikolouzakis TK, Stivaktakis PD, Kalogeraki A, Liesivuori J, Tzanakakis G, et al: Genotoxic, cytotoxic, and cytopathological effects in rats exposed for 18 months to a mixture of 13 chemicals in doses below NOAEL levels. Toxicol Lett. 316:154–170. 2019.PubMed/NCBI View Article : Google Scholar
25	Tsatsakis A, Tyshko NV, Docea AO, Shestakova SI, Sidorova YS, Petrov NA, Zlatian O, Mach M, Hartung T and Tutelyan VA: The effect of chronic vitamin deficiency and long term very low dose exposure to 6 pesticides mixture on neurological outcomes - A real-life risk simulation approach. Toxicol Lett. 315:96–106. 2019.PubMed/NCBI View Article : Google Scholar
26	Tsatsakis AM, Docea AO, Calina D, Buga AM, Zlatian O, Gutnikov S, Kostoff RN and Aschner M: Hormetic Neurobehavioral effects of low dose toxic chemical mixtures in real-life risk simulation (RLRS) in rats. Food Chem Toxicol. 125:141–149. 2019.PubMed/NCBI View Article : Google Scholar
27	Docea AO, Gofita E, Goumenou M, Calina D, Rogoveanu O, Varut M, Olaru C, Kerasioti E, Fountoucidou P, Taitzoglou I, et al: Six months exposure to a real life mixture of 13 chemicals' below individual NOAELs induced non monotonic sex-dependent biochemical and redox status changes in rats. Food Chem Toxicol. 115:470–481. 2018.PubMed/NCBI View Article : Google Scholar