Open Access

Small-size silver nanoparticles stimulate neutrophil oxidative burst through an increase of intracellular calcium levels

  • Authors:
    • Marisa Freitas
    • Mariana Lucas
    • Adelaide Sousa
    • Tânia Soares
    • Daniela Ribeiro
    • Félix Carvalho
    • Eduarda Fernandes
  • View Affiliations

  • Published online on: April 21, 2020     https://doi.org/10.3892/wasj.2020.46
  • ArticleNumber: 5
  • Copyright: © Freitas et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Despite the widespread use of silver nanoparticles (AgNPs) in several daily consumer products, the putative human risks resulting from continuous exposure remain elusive, particularly with what concerns the reactivity of the immune system and the resulting pro-inflammatory effects. Neutrophils are considered one of the first and primary cell types that process systemic nanoparticles, mediating the host inflammatory and immunological response, involving the production of reactive oxygen and nitrogen species. The main objective of the present study was to evaluate the effect of polyvinylpyrrolidone (PVP) and citrate-coated AgNPs of different sizes (5, 10 and 50 nm) on oxidative burst, calcium levels and the viability of human neutrophils. It was observed that the effects of AgNPs were size and coating-dependent. The citrate-coated and 5-nm-sized AgNPs were the most cytotoxic and potent inducers of neutrophil oxidative burst. It was observed that AgNPs induced an increase in intracellular calcium levels, which are involved in the activation of protein kinase C (PKC), resulting in the assembly of NADPH oxidase subunits with the subsequent production of reactive oxygen species. This excessive production of reactive oxygen species most probably contributes to the AgNP-induced decrease in neutrophil viability. Overall, the data of the present study contribute to a better understanding of the pro-inflammatory mechanisms associated with the use of AgNPs.
View Figures
View References

Related Articles

Journal Cover

May 2020
Volume 2 Issue 3

Print ISSN: 2632-2900
Online ISSN:2632-2919

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Freitas, M., Lucas, M., Sousa, A., Soares, T., Ribeiro, D., Carvalho, F., & Fernandes, E. (2020). Small-size silver nanoparticles stimulate neutrophil oxidative burst through an increase of intracellular calcium levels. World Academy of Sciences Journal, 2, 5. https://doi.org/10.3892/wasj.2020.46
MLA
Freitas, M., Lucas, M., Sousa, A., Soares, T., Ribeiro, D., Carvalho, F., Fernandes, E."Small-size silver nanoparticles stimulate neutrophil oxidative burst through an increase of intracellular calcium levels". World Academy of Sciences Journal 2.3 (2020): 5.
Chicago
Freitas, M., Lucas, M., Sousa, A., Soares, T., Ribeiro, D., Carvalho, F., Fernandes, E."Small-size silver nanoparticles stimulate neutrophil oxidative burst through an increase of intracellular calcium levels". World Academy of Sciences Journal 2, no. 3 (2020): 5. https://doi.org/10.3892/wasj.2020.46