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Isotope effect of 13C‑enriched testosterone on human cells

  • Authors:
    • Mengqi Zhang
    • Xinchen Wu
    • Wenning Yang
    • Tengfei Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Harlem Hospital Center, Affiliated with Columbia University, New York, NY 10037, USA, Sanbo Brain Hospital, Capital Medical University, Beijing 100093, P.R. China, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 9
    |
    Published online on: February 16, 2022
       https://doi.org/10.3892/wasj.2022.144
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Abstract

The present study investigated the carbon isotope effect of carbon‑13 (13C)‑enriched testosterone on human cells; studies on the carbon isotope effects of a bioactive compound are limited. For this purpose, human osteoblasts, aortic endothelial cells and umbilical vein endothelial cells were treated with testosterone and 13C‑enriched testosterone. The cell growth rates and bioactivities of osteoblasts were measured. At physiological concentrations, testosterone promoted cell proliferation, whereas 13C‑enriched testosterone exerted a neutral and concentration‑independent effect on the cells. On the whole, the findings of the present study demonstrated that 13C‑enriched testosterone exerted a significant isotope effect on human cells; this needs to be taken into account in stable isotope‑based biology research.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang M, Wu X, Yang W and Zhang T: Isotope effect of <sup>13</sup>C‑enriched testosterone on human cells. World Acad Sci J 4: 9, 2022.
APA
Zhang, M., Wu, X., Yang, W., & Zhang, T. (2022). Isotope effect of <sup>13</sup>C‑enriched testosterone on human cells. World Academy of Sciences Journal, 4, 9. https://doi.org/10.3892/wasj.2022.144
MLA
Zhang, M., Wu, X., Yang, W., Zhang, T."Isotope effect of <sup>13</sup>C‑enriched testosterone on human cells". World Academy of Sciences Journal 4.2 (2022): 9.
Chicago
Zhang, M., Wu, X., Yang, W., Zhang, T."Isotope effect of <sup>13</sup>C‑enriched testosterone on human cells". World Academy of Sciences Journal 4, no. 2 (2022): 9. https://doi.org/10.3892/wasj.2022.144
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang M, Wu X, Yang W and Zhang T: Isotope effect of <sup>13</sup>C‑enriched testosterone on human cells. World Acad Sci J 4: 9, 2022.
APA
Zhang, M., Wu, X., Yang, W., & Zhang, T. (2022). Isotope effect of <sup>13</sup>C‑enriched testosterone on human cells. World Academy of Sciences Journal, 4, 9. https://doi.org/10.3892/wasj.2022.144
MLA
Zhang, M., Wu, X., Yang, W., Zhang, T."Isotope effect of <sup>13</sup>C‑enriched testosterone on human cells". World Academy of Sciences Journal 4.2 (2022): 9.
Chicago
Zhang, M., Wu, X., Yang, W., Zhang, T."Isotope effect of <sup>13</sup>C‑enriched testosterone on human cells". World Academy of Sciences Journal 4, no. 2 (2022): 9. https://doi.org/10.3892/wasj.2022.144
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