Expression of microRNAs in gastric cancerous tissues and their association with <em>Helicobacter pylori</em> and Epstein‑Barr virus infections

Rihane,Fatima Ezzahra; Erguibi,Driss; Abumsimir,Berjas; Charoute,Hicham; Chehab,Farid; Ennaji,Moulay Mustapha

doi:10.3892/wasj.2022.171

Expression of microRNAs in gastric cancerous tissues and their association with Helicobacter pylori and Epstein‑Barr virus infections

Authors:
- Fatima Ezzahra Rihane
- Driss Erguibi
- Berjas Abumsimir
- Hicham Charoute
- Farid Chehab
- Moulay Mustapha Ennaji
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Affiliations: Laboratory of Genetic and Molecular Pathology, Faculty of Medicine and Pharmacy of Casablanca, University Hassan II of Casablanca, Casablanca 20250, Morocco, Service of Digestive Cancers Surgery and Liver Transplant, Department of Surgery, Ibn Rochd University Hospital Center, Faculty of Medicine and Pharmacy of Casablanca, University Hassan II of Casablanca, Casablanca 20250, Morocco, Pharmacological and Diagnostic Research Centre (PDRC), Department of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Al‑Ahliyya Amman University (AAU), Amman 19328, Jordan, Research Unit of Epidemiology, Biostatistics and Bioinformatics, Pasteur Institute of Morocco, Casablanca 20360, Morocco, Laboratory of Virology, Microbiology, Quality, Biotechnologies/Ecotoxicology and Biodiversity, Faculty of Sciences and Technologies Mohammedia, University Hassan II of Casablanca, Mohammedia 20650, Morocco
Published online on: September 13, 2022 https://doi.org/10.3892/wasj.2022.171
Article Number: 36
Copyright: © Rihane et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gastric cancer (GC) is a serious public health concern, being one of the most frequent types of cancer worldwide, and the most lethal of all gastrointestinal cancers. Among the numerous causes of the occurrence and progression of GC, are infections of the gastric epithelium by microbial agents, such as Epstein‑Barr virus and/or Helicobacter pylori (H. pylori). Recent findings have shed new light on the involvement of a class of non‑coding RNAs, known as microRNAs (miRNAs/miRs) that are differentially expressed in GC tissues. Among these miRNAs, miR‑21, miR‑223 and miR‑19, appear to play a crucial role in tumorigenesis. The present study aimed to investigate the differential expression of miR‑21, miR‑223 and miR‑19 in GC tissues and their association with Epstein‑Barr virus and H. pylori infections. The expression levels of miR‑21, miR‑223 and miR‑19 in 70 samples of cancerous and adjacent normal tissues from patients with GC were detected using reverse transcription‑quantitative polymerase chain reaction. The clinical relevance was then statistically analyzed. The results revealed the overexpression of the three miRNAs, miR‑21, miR‑223 and miR‑19, in tumor tissues compared to the corresponding normal tissues. In addition, the upregulation of the mentioned miRNAs in GC tissues was also significantly associated with Epstein‑Barr virus and H. pylori infections, as well as with aggressive clinicopathological features. On the whole, the findings of the present study indicate the potential of miR‑21, miR‑223 and miR‑19 for use as novel diagnostic, prognostic and predictive biomarkers for gastric tumors. However, further randomized studies involving diverse populations and functional assessments are required to extend and confirm these findings.

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