Comparative evaluation of the efficacy of the mechanical agitation‑assisted decalcification of human permanent teeth: A histological analysis

Shahid,Mohammad; Shetty,Preethesh; Paul,Tony; Bhat,Raksha

doi:10.3892/wasj.2023.203

September-October 2023 Volume 5 Issue 5

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September-October 2023 Volume 5 Issue 5

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Comparative evaluation of the efficacy of the mechanical agitation‑assisted decalcification of human permanent teeth: A histological analysis

Authors:
- Mohammad Shahid
- Preethesh Shetty
- Tony Paul
- Raksha Bhat
View Affiliations / Copyright

Affiliations: A.J. Shetty Institute of Dental Sciences, Mangaluru, Karnataka 575004, India, Department of Conservative Dentistry and Endodontics, AB Shetty Memorial Institute of Dental Sciences (ABSMIDS), Nitte (Deemed to be University), Mangaluru, Karnataka 575018, India, Germans Trias I Pujol Research Institute (IGTP), Badalona, 08916 Barcelona, Spain

Copyright: © Shahid et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].
Article Number: 26
|
Published online on: September 26, 2023

https://doi.org/10.3892/wasj.2023.203
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Abstract

The decalcification of teeth constitutes a persistent challenge for pathology and histology research laboratories. The conflict concerning the duration of the procedure and the fineness of the sections render it fundamentally challenging. The present study aimed to introduce a novel method for decalcifying teeth using a magnetic agitator. For this purpose, 192 non‑carious premolars extracted for orthodontic treatment were collected and fixed immediately with 10% formalin. The apical tips of all the samples were removed. A total of four decalcifying agents (DAs) were selected for decalcification: 5% Nitric acid (group I), 10% HCl (group II), 10% formic acid (group III) and 14% EDTA (group IV). The samples were decalcified separately by the same solution, one with and one without agitation with a magnetic agitator. The solutions were changed daily to accelerate decalcification. The decalcification rate (DR) was higher in group I, followed by groups II, III and IV. There was a statistically significant difference between the groups as regards the DR. Agitation significantly reduced the decalcification time for all the DAs used. Tissue preservation following decalcification was sufficient with the group IV solutions, average with those used in groups II and III, and poor with the group I solutions. Tissue preservation was improved in the sections subjected to agitation than in those without. On the whole, the results of the present study suggest that 14% EDTA can decalcify at an accepted rate and provides satisfactory tissue details for decalcifying healthy premolars with an agitation speed at 100 rpm. However, further studies are required to rule out the effects of this DA on carious, malformed teeth associated with pathologies, normal and pathological bone.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Comparative evaluation of the efficacy of the mechanical agitation‑assisted decalcification of human permanent teeth: A histological analysis

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