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Article Open Access

Detection of SARS‑CoV‑2 Delta, Omicron and XBB variant using colorimetric reverse‑transcription loop‑mediated isothermal amplification and specific primers

  • Authors:
    • Kheng Oon Low
    • Nurhezreen Md Iqbal
    • Azrin Ahmad
    • Nor Azfa Johari
  • View Affiliations / Copyright

    Affiliations: Synthetic Biology and Genetic Engineering Division, Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, 43000 Kajang, Selangor, Malaysia, Advanced Genomic and Bioinformatics Division, Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, 43000 Kajang, Selangor, Malaysia, Structural Biology and Functional Omics Division, Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, 43000 Kajang, Selangor, Malaysia
    Copyright: © Low et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].
  • Article Number: 50
    |
    Published online on: March 27, 2025
       https://doi.org/10.3892/wasj.2025.338
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Abstract

Even with global vaccination efforts, COVID‑19 continues due to the emergent of new and more transmissible variants of severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) that evade immunity. Monitoring these variants is costly, often requiring whole‑genome sequencing. Therefore, there is a need for more cost‑effective and rapid alternatives for the identification of variant‑specific samples. Reverse‑transcription loop‑mediated isothermal amplification (RT‑LAMP) provides a more affordable and rapid method for detecting SARS‑CoV‑2 compared to other molecular tests. The present study designed colorimetric RT‑LAMP assays that target mutations associated with specific SARS‑CoV‑2 lineages. These assays were tested on 347 confirmed SARS‑CoV‑2 RNA samples. The results revealed promising accuracy: 92.18% for Omicron‑specific primers, followed by 80.64% for XBB and 71.57% for Delta. This assay effectively distinguishes the variants of concern, Delta, Omicron and XBB, without the use of costly equipment/facilities and does not require the specialized training of personnel. This development supports the use of RT‑LAMP as a valuable tool in healthcare systems battling COVID‑19.
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Copy and paste a formatted citation
Spandidos Publications style
Low K, Md Iqbal N, Ahmad A and Johari N: Detection of SARS‑CoV‑2 Delta, Omicron and XBB variant using colorimetric reverse‑transcription loop‑mediated isothermal amplification and specific primers. World Acad Sci J 7: 50, 2025.
APA
Low, K., Md Iqbal, N., Ahmad, A., & Johari, N. (2025). Detection of SARS‑CoV‑2 Delta, Omicron and XBB variant using colorimetric reverse‑transcription loop‑mediated isothermal amplification and specific primers. World Academy of Sciences Journal, 7, 50. https://doi.org/10.3892/wasj.2025.338
MLA
Low, K., Md Iqbal, N., Ahmad, A., Johari, N."Detection of SARS‑CoV‑2 Delta, Omicron and XBB variant using colorimetric reverse‑transcription loop‑mediated isothermal amplification and specific primers". World Academy of Sciences Journal 7.4 (2025): 50.
Chicago
Low, K., Md Iqbal, N., Ahmad, A., Johari, N."Detection of SARS‑CoV‑2 Delta, Omicron and XBB variant using colorimetric reverse‑transcription loop‑mediated isothermal amplification and specific primers". World Academy of Sciences Journal 7, no. 4 (2025): 50. https://doi.org/10.3892/wasj.2025.338
Copy and paste a formatted citation
x
Spandidos Publications style
Low K, Md Iqbal N, Ahmad A and Johari N: Detection of SARS‑CoV‑2 Delta, Omicron and XBB variant using colorimetric reverse‑transcription loop‑mediated isothermal amplification and specific primers. World Acad Sci J 7: 50, 2025.
APA
Low, K., Md Iqbal, N., Ahmad, A., & Johari, N. (2025). Detection of SARS‑CoV‑2 Delta, Omicron and XBB variant using colorimetric reverse‑transcription loop‑mediated isothermal amplification and specific primers. World Academy of Sciences Journal, 7, 50. https://doi.org/10.3892/wasj.2025.338
MLA
Low, K., Md Iqbal, N., Ahmad, A., Johari, N."Detection of SARS‑CoV‑2 Delta, Omicron and XBB variant using colorimetric reverse‑transcription loop‑mediated isothermal amplification and specific primers". World Academy of Sciences Journal 7.4 (2025): 50.
Chicago
Low, K., Md Iqbal, N., Ahmad, A., Johari, N."Detection of SARS‑CoV‑2 Delta, Omicron and XBB variant using colorimetric reverse‑transcription loop‑mediated isothermal amplification and specific primers". World Academy of Sciences Journal 7, no. 4 (2025): 50. https://doi.org/10.3892/wasj.2025.338
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