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Erythrocyte sedimentation reaction order, neutrophil‑lymphocyte ratio and platelet‑lymphocyte ratio in patients with breast cancer: Development of an early detection method for cancer

  • Authors:
    • Ngadikun
    • Usi Sukorini
    • Kuncoro Asih Nugroho
    • Metamalik Pasala
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, Faculty of Medicine, Public Health and Nursing Gadjah Mada University, Yogyakarta 55281, Indonesia, Department of Clinical Pathology, Faculty of Medicine, Public Health and Nursing Gadjah Mada University, Yogyakarta 55281, Indonesia, Department of Physics Education, Yogyakarta State University, Yogyakarta 55281, Indonesia, Faculty of Pharmacy, Gajah Mada University, Yogyakarta 55281, Indonesia
    Copyright: © Ngadikun et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].
  • Article Number: 94
    |
    Published online on: July 31, 2025
       https://doi.org/10.3892/wasj.2025.382
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Abstract

One of the main causes of mortality due to cancer is breast cancer (BC). The high incidence and mortality rates are partly due to the absence of the early detection of this cancer. Moreover, the investigation of biomarkers which play a key role in the prognosis of the disease is critical. The present study aimed to develop a simple early method for detecting BC with three parameters, namely the erythrocyte sedimentation rate order (ESRO), neutrophil‑lymphocyte ratio (NLR) and platelet‑lymphocyte ratio (PLR) that indicate the presence of biomarkers in patients with BC. These parameters were evaluated in 27 patients with BC using receiver‑operating characteristic (ROC) curve analysis and were then compared to the normal group (n=29). The present study calculated ROC, the area under the curve (AUC), sensitivity, and specificity values to determine the ESRO, NLR and PLR levels that can most efficiently differentiate between patients with BC and control subjects. The ESRO parameter exhibited a better performance than NLR and PLR in differentiating patients with breast cancer and normal subjects from whole blood samples (AUC values were 82, 65 and 73%, respectively), for the selected cut off points of 6.075, 2.86 and 216.0, respectively. Thus, it can be concluded that ESRO performs better as inflammatory parameter to reflect the presence of BC compared to NLR and PLR.
View Figures

Figure 1

Scatter diagram (left) and boxplot
mean (right) of (A) ESRO, (B) NLR and (C) PLR for BC patients and
normal subjects. ESRO, erythrocyte sedimentation rate order; NLR,
neutrophil-lymphocyte ratio; PLR, platelet-lymphocyte ratio.

Figure 2

Receiver operating characteristic and
area under curve values of biomarkers differentiating between
patients with breast cancer and normal subjects. ESRO, erythrocyte
sedimentation rate order; NLR, neutrophil-lymphocyte ratio; PLR,
platelet-lymphocyte ratio.
View References

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Spandidos Publications style
Ngadikun , Sukorini U, Nugroho KA and Pasala M: Erythrocyte sedimentation reaction order, neutrophil‑lymphocyte ratio and platelet‑lymphocyte ratio in patients with breast cancer: Development of an early detection method for cancer. World Acad Sci J 7: 94, 2025.
APA
Ngadikun, , Sukorini, U., Nugroho, K.A., & Pasala, M. (2025). Erythrocyte sedimentation reaction order, neutrophil‑lymphocyte ratio and platelet‑lymphocyte ratio in patients with breast cancer: Development of an early detection method for cancer. World Academy of Sciences Journal, 7, 94. https://doi.org/10.3892/wasj.2025.382
MLA
Ngadikun, , Sukorini, U., Nugroho, K. A., Pasala, M."Erythrocyte sedimentation reaction order, neutrophil‑lymphocyte ratio and platelet‑lymphocyte ratio in patients with breast cancer: Development of an early detection method for cancer". World Academy of Sciences Journal 7.5 (2025): 94.
Chicago
Ngadikun, , Sukorini, U., Nugroho, K. A., Pasala, M."Erythrocyte sedimentation reaction order, neutrophil‑lymphocyte ratio and platelet‑lymphocyte ratio in patients with breast cancer: Development of an early detection method for cancer". World Academy of Sciences Journal 7, no. 5 (2025): 94. https://doi.org/10.3892/wasj.2025.382
Copy and paste a formatted citation
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Spandidos Publications style
Ngadikun , Sukorini U, Nugroho KA and Pasala M: Erythrocyte sedimentation reaction order, neutrophil‑lymphocyte ratio and platelet‑lymphocyte ratio in patients with breast cancer: Development of an early detection method for cancer. World Acad Sci J 7: 94, 2025.
APA
Ngadikun, , Sukorini, U., Nugroho, K.A., & Pasala, M. (2025). Erythrocyte sedimentation reaction order, neutrophil‑lymphocyte ratio and platelet‑lymphocyte ratio in patients with breast cancer: Development of an early detection method for cancer. World Academy of Sciences Journal, 7, 94. https://doi.org/10.3892/wasj.2025.382
MLA
Ngadikun, , Sukorini, U., Nugroho, K. A., Pasala, M."Erythrocyte sedimentation reaction order, neutrophil‑lymphocyte ratio and platelet‑lymphocyte ratio in patients with breast cancer: Development of an early detection method for cancer". World Academy of Sciences Journal 7.5 (2025): 94.
Chicago
Ngadikun, , Sukorini, U., Nugroho, K. A., Pasala, M."Erythrocyte sedimentation reaction order, neutrophil‑lymphocyte ratio and platelet‑lymphocyte ratio in patients with breast cancer: Development of an early detection method for cancer". World Academy of Sciences Journal 7, no. 5 (2025): 94. https://doi.org/10.3892/wasj.2025.382
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