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![Elution profile of milk-clotting
enzyme from Penicillium purpurescens purified by acetone
fractionation, then DEAE-cellulose ion-exchange chromatography.
Protein content was monitored at 280 nm, and milk-clotting activity
was assayed in collected fractions. Active MCE fractions were
observed at (NaCl concentration range] 0.05 to 0.1 mM), indicating
successful separation from non-active proteins.](/article_images/wasj/7/6/wasj-07-06-00399-g06.jpg)
