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Multidrug-resistance proteins and glucose transporter expression in cutaneous squamous cell carcinoma and adjacent healthy tissue in hairless mice

  • Authors:
    • George Parthymos
    • Maria Kyriazi
    • Christina Barda
    • Andreas Vitsos
    • Michail Christou Rallis
  • View Affiliations / Copyright

    Affiliations: Research Unit of Dermatopharmacology-Cosmetology, Laboratory of Pharmaceutical Technology, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece
    Copyright: © Parthymos et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].
  • Article Number: 51
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    Published online on: April 27, 2026
       https://doi.org/10.3892/wasj.2026.466
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Abstract

The present study examined the expression and potential roles of multi-drug resistance P-glycoprotein (P-gp), multi-drug resistance-associated protein (MRP) and glucose transporter type 1 (GLUT-1) in cutaneous squamous cell carcinoma (cSCC). Despite their recognized involvement in drug resistance, the specific contributions of these transporters to cSCC remain poorly understood. Using a cSCC hairless mouse model and through immunochemical analysis, the expression of P-gp, MRP-1 and GLUT-1 in cancerous and normal skin tissues was examined. The findings revealed that all assessed transporters were expressed across all skin tissues; however, the expression levels were notably higher in tumor and tumor-adjacent areas compared with normal tissues. This differential expression pattern suggests a complex interaction between these transporters and cSCC development, highlighting the nuanced nature of protein expression variations in cancerous and surrounding tissues. Despite the limited sample size, statistically significant variations in expression levels were observed. These findings may serve as a basis for hypothesis generation regarding the molecular mechanisms involved in cSCC development and progression, laying the groundwork for future investigations.
View Figures

Figure 1

Multi-drug resistance-associated
protein 1 measurements (n=5): Values are presented as the mean ±
SD. Within-subject comparisons were made using the Friedman test
with Durbin-Conover post hoc pairwise tests (adjusted P-values):
global χ2=3.89, P=0.143; cancer vs. adjacent
P_adj=0.857; cancer vs. distant P_adj=0.075; adjacent vs. distant
p_adj=0.100. No pairwise contrast reached statistical significance
following adjustment.

Figure 2

P-glycoprotein expression levels
(n=5): Values are presented as the mean ± SD. Friedman test: global
χ2=5.20, P=0.074. Post hoc pairwise (adjusted): adjacent
> distant *P_adj=0.021, cancer vs. distant
P_adj=0.076 (borderline trend), cancer vs. adjacent P_adj=0.438.
*P_adj<0.05.

Figure 3

Glucose transporter 1 measurements
(n=5): Values are presented as the mean ± SD. Friedman test: global
χ2=5.44, P=0.066. Post hoc pairwise (adjusted): cancer
> distant *P_adj=0.041, adjacent > distant
*P_adj=0.021, cancer vs. adjacent
*P_adj=0.670. *P_adj<0.05.
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Spandidos Publications style
Parthymos G, Kyriazi M, Barda C, Vitsos A and Rallis MC: Multidrug-resistance proteins and glucose transporter expression in cutaneous squamous cell carcinoma and adjacent healthy tissue in hairless mice. World Acad Sci J 8: 51, 2026.
APA
Parthymos, G., Kyriazi, M., Barda, C., Vitsos, A., & Rallis, M.C. (2026). Multidrug-resistance proteins and glucose transporter expression in cutaneous squamous cell carcinoma and adjacent healthy tissue in hairless mice. World Academy of Sciences Journal, 8, 51. https://doi.org/10.3892/wasj.2026.466
MLA
Parthymos, G., Kyriazi, M., Barda, C., Vitsos, A., Rallis, M. C."Multidrug-resistance proteins and glucose transporter expression in cutaneous squamous cell carcinoma and adjacent healthy tissue in hairless mice". World Academy of Sciences Journal 8.3 (2026): 51.
Chicago
Parthymos, G., Kyriazi, M., Barda, C., Vitsos, A., Rallis, M. C."Multidrug-resistance proteins and glucose transporter expression in cutaneous squamous cell carcinoma and adjacent healthy tissue in hairless mice". World Academy of Sciences Journal 8, no. 3 (2026): 51. https://doi.org/10.3892/wasj.2026.466
Copy and paste a formatted citation
x
Spandidos Publications style
Parthymos G, Kyriazi M, Barda C, Vitsos A and Rallis MC: Multidrug-resistance proteins and glucose transporter expression in cutaneous squamous cell carcinoma and adjacent healthy tissue in hairless mice. World Acad Sci J 8: 51, 2026.
APA
Parthymos, G., Kyriazi, M., Barda, C., Vitsos, A., & Rallis, M.C. (2026). Multidrug-resistance proteins and glucose transporter expression in cutaneous squamous cell carcinoma and adjacent healthy tissue in hairless mice. World Academy of Sciences Journal, 8, 51. https://doi.org/10.3892/wasj.2026.466
MLA
Parthymos, G., Kyriazi, M., Barda, C., Vitsos, A., Rallis, M. C."Multidrug-resistance proteins and glucose transporter expression in cutaneous squamous cell carcinoma and adjacent healthy tissue in hairless mice". World Academy of Sciences Journal 8.3 (2026): 51.
Chicago
Parthymos, G., Kyriazi, M., Barda, C., Vitsos, A., Rallis, M. C."Multidrug-resistance proteins and glucose transporter expression in cutaneous squamous cell carcinoma and adjacent healthy tissue in hairless mice". World Academy of Sciences Journal 8, no. 3 (2026): 51. https://doi.org/10.3892/wasj.2026.466
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