Synthetic polyphosphate inhibits endogenous coagulation and platelet aggregation in vitro

Yang,Xiaoyang; Wan,Mengjie; Liang,Ting; Peng,Minyuan; Chen,Fangping

doi:10.3892/br.2016.816

Synthetic polyphosphate inhibits endogenous coagulation and platelet aggregation in vitro

Authors:
- Xiaoyang Yang
- Mengjie Wan
- Ting Liang
- Minyuan Peng
- Fangping Chen
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Affiliations: Department of Hematology, Haikou Municipal Hospital, Affiliated Haikou Hospital of Central South University, Haikou, Hainan 570208, P.R. China, Department of Hematology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
Published online on: November 23, 2016 https://doi.org/10.3892/br.2016.816
Pages: 57-62

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Abstract

Platelet-derived polyphosphate has previously been indicated to induce coagulation. However, industrially synthesized polyphosphate has been found to have different effects from those of the platelet-derived form. The present study investigated whether synthetic sodium polyphosphate inhibits coagulation using routine coagulation tests and thromboelastography. Synthetic polyphosphate was found to inhibit adenosine diphosphate-, epinephrine-, arachidonic acid-, ristocetin-, thrombin-, oxytocin- and pituitrin-induced platelet aggregation. The effects of synthetic polyphosphate in clotting inhibition were revealed by the analysis of clotting factor activity and platelet aggregation tests. Synthetic polyphosphate may inhibit platelet aggregation by reducing platelet calcium levels, as indicated by the results of flow cytometric analysis and high-throughput fluorescent screening. Furthermore, analysis of thromboxane (TX)B2 by ELISA indicated that synthetic polyphosphate reduces platelet aggregation by inhibiting the TXA2 signaling pathway. In conclusion, synthetic polyphosphate inhibits clotting factor activity and endogenous coagulation by reducing the levels of calcium ions and TXA2 to curb platelet aggregation.

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