Open Access

Aberrant expression of interleukin-10 and activation-induced cytidine deaminase in B cells from patients with Behçet's disease

  • Authors:
    • Jeong-Yun Yoon
    • Yeojin Lee
    • Seong-Lan Yu
    • Hee-Kyung Yoon
    • Ha-Yan Park
    • Chung-Il Joung
    • Seok-Rae Park
    • Mihye Kwon
    • Jaeku Kang
  • View Affiliations

  • Published online on: October 4, 2017     https://doi.org/10.3892/br.2017.996
  • Pages:520-526
  • Copyright: © Yoon et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Despite extensive studies, the pathogenesis of Behçet's disease (BD) remains unclear. In particular, the roles of B cells in patients with BD have not been elucidated. Activation-induced cytidine deaminase (AID) is a critical enzyme for immunoglobulin (Ig) heavy chain class switching and somatic hypermutation in B cells and the abnormal expression of AID in various immune conditions has previously been studied. B10 cells, an interleukin (IL)-10-secreting subset of regulatory B cells, function to downregulate inflammation and autoimmunity. Thus, in the present study, the relevance of B cells in patients with BD was investigated. The plasma levels of IL-10 and IgA and the proportions of cluster of differentiation (CD)43+ B cells, excluding naïve B cells, were measured in 16 patients with BD and 16 age- and sex-matched healthy controls (HCs). Additionally, the mRNA levels of IL-10 and AID were assessed in B cells from fresh peripheral blood samples of the BD patients and HCs. The plasma level of IL-10 in patients with BD did not differ significantly from that in HCs. Similarly, there was no significant difference in the plasma level of IgA, although a slight increase was observed in patients with BD compared with that in HCs. There were no differences in CD43+CD19+ B cell numbers between patients with BD and HCs. However, IL-10 mRNA levels were significantly reduced (P<0.05), while AID mRNA levels were significantly increased (P<0.01) in the B cells of patients with BD compared with those in HCs. These results provide insight into the role of B cells in patients with BD.

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December 2017
Volume 7 Issue 6

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APA
Yoon, J., Lee, Y., Yu, S., Yoon, H., Park, H., Joung, C. ... Kang, J. (2017). Aberrant expression of interleukin-10 and activation-induced cytidine deaminase in B cells from patients with Behçet's disease. Biomedical Reports, 7, 520-526. https://doi.org/10.3892/br.2017.996
MLA
Yoon, J., Lee, Y., Yu, S., Yoon, H., Park, H., Joung, C., Park, S., Kwon, M., Kang, J."Aberrant expression of interleukin-10 and activation-induced cytidine deaminase in B cells from patients with Behçet's disease". Biomedical Reports 7.6 (2017): 520-526.
Chicago
Yoon, J., Lee, Y., Yu, S., Yoon, H., Park, H., Joung, C., Park, S., Kwon, M., Kang, J."Aberrant expression of interleukin-10 and activation-induced cytidine deaminase in B cells from patients with Behçet's disease". Biomedical Reports 7, no. 6 (2017): 520-526. https://doi.org/10.3892/br.2017.996