1α,25‑Dihydroxyvitamin D3 inhibits the differentiation and bone resorption by osteoclasts generated from Wistar rat bone marrow‑derived macrophages

Wang,Dong; Gu,Jian‑Hong; Chen,Yang; Zhao,Hong‑Yan; Liu,Wei; Song,Rui‑Long; Bian,Jian‑Chun; Liu,Xue‑Zhong; Yuan,Yan; Liu,Zong‑Ping

doi:10.3892/etm.2015.2632

1α,25‑Dihydroxyvitamin D3 inhibits the differentiation and bone resorption by osteoclasts generated from Wistar rat bone marrow‑derived macrophages

Authors:
- Dong Wang
- Jian‑Hong Gu
- Yang Chen
- Hong‑Yan Zhao
- Wei Liu
- Rui‑Long Song
- Jian‑Chun Bian
- Xue‑Zhong Liu
- Yan Yuan
- Zong‑Ping Liu
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Affiliations: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China
Published online on: July 8, 2015 https://doi.org/10.3892/etm.2015.2632
Pages: 1039-1044

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Abstract

The steroid hormone 1α,25‑dihydroxyvitamin D3 [1α,25‑(OH)2D3] plays an important role in maintaining a balance in calcium and bone metabolism. To study the effects of 1α,25‑(OH)2D3 on osteoclast (OC) formation and bone resorption, OC differentiation was induced in bone marrow‑derived mononuclear cells from Wistar rats with the addition of macrophage colony stimulating factor and receptor activator for nuclear factor‑κB ligand in vitro. Cells were then treated with 1α,25‑(OH)2D3 at 10‑9, 10‑8 or 10‑7 mol/l. OCs were identified using tartrate‑resistant acid phosphatase staining and activity was monitored in the absorption lacunae by scanning electron microscopy. Expression levels of functional proteins associated with bone absorption, namely carbonic anhydrase II, cathepsin K and matrix metalloproteinase‑9 were evaluated by western blot analysis. The results showed that 1α,25‑(OH)2D3 inhibited the formation and activation of OCs in a dose‑dependent manner and downregulated the expression levels of bone absorption‑associated proteins.

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1	Qu X, Zhai Z, Liu X, et al: Dioscin inhibits osteoclast differentiation and bone resorption through down-regulating the Akt signaling cascades. Biochem Biophys Res Commun. 443:658–665. 2014. View Article : Google Scholar : PubMed/NCBI
2	Lieben L, Carmeliet G and Masuyama R: Calcemic actions of vitamin D: Effects on the intestine, kidney and bone. Best Pract Res Clin Endocrinol Metab. 25:561–572. 2011. View Article : Google Scholar : PubMed/NCBI
3	Lieben L and Carmeliet G: Vitamin D signaling in osteocytes: Effects on bone and mineral homeostasis. Bone. 54:237–243. 2013. View Article : Google Scholar : PubMed/NCBI
4	Kogawa M, Anderson PH, Findlay DM, et al: The metabolism of 25-(OH)vitamin D3 by osteoclasts and their precursors regulates the differentiation of osteoclasts. J Steroid Biochem Mol Biol. 121:277–280. 2010. View Article : Google Scholar : PubMed/NCBI
5	Uchiyama Y, Higuchi Y, Takeda S, et al: ED-71, a vitamin D analog, is a more potent inhibitor of bone resorption than alfacalcidol in an estrogen-deficient rat model of osteoporosis. Bone. 30:582–588. 2002. View Article : Google Scholar : PubMed/NCBI
6	Song R, Gu J, Liu X, Zhu J, Wang Q, Gao Q, Zhang J, Cheng L, Tong X, Qi X, et al: Inhibition of osteoclast bone resorption activity through osteoprotegerin-induced damage of the sealing zone. Int J Mol Med. 34:856–862. 2014.PubMed/NCBI
7	Gu JH, Tong XS, Chen GH, et al: Regulation of matrix metalloproteinase-9 protein expression by 1α,25-(OH)₂D₃ during osteoclast differentiation. J Vet Sci. 15:133–140. 2014. View Article : Google Scholar : PubMed/NCBI
8	Teitelbaum SL: Osteoclasts: What do they do and how do they do it? Am J Pathol. 170:427–435. 2007. View Article : Google Scholar : PubMed/NCBI
9	Brinkmann J, Hefti T, Schlottig F, et al: Response of osteoclasts to titanium surfaces with increasing surface roughness: An in vitro study. Biointerphases. 7:342012. View Article : Google Scholar : PubMed/NCBI
10	Suda T, Takahashi F and Takahashi N: Bone effects of vitamin D - Discrepancies between in vivo and in vitro studies. Arch Biochem Biophys. 523:22–29. 2012. View Article : Google Scholar : PubMed/NCBI
11	Takasu H, Sugita A, Uchiyama Y, et al: c-Fos protein as a target of anti-osteoclastogenic action of vitamin D and synthesis of new analogs. J Clin Invest. 116:528–535. 2006. View Article : Google Scholar : PubMed/NCBI
12	Zhu K, Gläser R and Mrowietz U: Vitamin D(3) and analogues modulate the expression of CSF-1 and its receptor in human dendritic cells. Biochem Biophys Res Commun. 297:1211–1217. 2002. View Article : Google Scholar : PubMed/NCBI
13	Medhora MM, Teitelbaum S, Chappel J, et al: 1 alpha, 25-dihydroxyvitamin D3 up-regulates expression of the osteoclast integrin alpha v beta 3. J Biol Chem. 268:1456–1461. 1993.PubMed/NCBI
14	Takahashi N, Yamana H, Yoshiki S, et al: Osteoclast-like cell formation and its regulation by osteotropic hormones in mouse bone marrow cultures. Endocrinology. 122:1373–1382. 1988. View Article : Google Scholar : PubMed/NCBI
15	DeLuca HF: History of the discovery of vitamin D and its active metabolites. Bonekey Rep. 3:4792014. View Article : Google Scholar : PubMed/NCBI
16	Arai F, Miyamoto T, Ohneda O, et al: Commitment and differentiation of osteoclast precursor cells by the sequential expression of c-Fms and receptor activator of nuclear factor κB (RANK) receptors. J Exp Med. 190:1741–1754. 1999. View Article : Google Scholar : PubMed/NCBI
17	Price CP, Kirwan A and Vader C: Tartrate-resistant acid phosphatase as a marker of bone resorption. Clin Chem. 41:641–643. 1995.PubMed/NCBI
18	Kim TH, Lee B, Kwon E, et al: 1,25-Dihydroxyvitamin D3 inhibits directly human osteoclastogenesis by down-regulation of the c-Fms and RANK expression. Joint Bone Spine. 80:307–314. 2013. View Article : Google Scholar : PubMed/NCBI
19	Väänänen HK, Liu YK, Lehenkari P and Uemara T: How do osteoclasts resorb bone? Mater Sci Eng C. 6:205–209. 1998. View Article : Google Scholar
20	Grüneberg S, Stubbs MT and Klebe G: Successful virtual screening for novel inhibitors of human carbonic anhydrase: Strategy and experimental confirmation. J Med Chem. 45:3588–3602. 2002. View Article : Google Scholar : PubMed/NCBI
21	Väänänen K: Mechanism of osteoclast mediated bone resorption - rationale for the design of new therapeutics. Adv Drug Deliv Rev. 57:959–971. 2005. View Article : Google Scholar : PubMed/NCBI
22	Xie L, Moroi Y, Hayashida S, et al: Cathepsin K-upregulation in fibroblasts promotes matrigel invasive ability of squamous cell carcinoma cells via tumor-derived IL-1α. J Dermatol Sci. 61:45–50. 2011. View Article : Google Scholar : PubMed/NCBI
23	Ishibashi O, Niwa S, Kadoyama K and Inui T: MMP-9 antisense oligodeoxynucleotide exerts an inhibitory effect on osteoclastic bone resorption by suppressing cell migration. Life Sci. 79:1657–1660. 2006. View Article : Google Scholar : PubMed/NCBI