A novel method for right one-lung ventilation modeling in rabbits

Xu,Ze‑Ping; Gu,Lian‑Bing; Bian,Qing‑Ming; Li,Peng‑Yi; Wang,Li‑Jun; Chen,Xiao‑Xiang; Zhang,Jing‑Yuan

doi:10.3892/etm.2016.3434

A novel method for right one-lung ventilation modeling in rabbits

Authors:
- Ze‑Ping Xu
- Lian‑Bing Gu
- Qing‑Ming Bian
- Peng‑Yi Li
- Li‑Jun Wang
- Xiao‑Xiang Chen
- Jing‑Yuan Zhang
View Affiliations

Affiliations: Department of Anesthesiology, Jiangsu Cancer Hospital, Jiangsu Cancer Institute, Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210009, P.R. China, Department of Gynecology, Jiangsu Cancer Hospital, Jiangsu Cancer Institute, Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210009, P.R. China, Department of Pathology, Jiangsu Cancer Hospital, Jiangsu Cancer Institute, Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210009, P.R. China
Published online on: June 7, 2016 https://doi.org/10.3892/etm.2016.3434
Pages: 1213-1219

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

There is no standard method by which to establish a right one-lung ventilation (OLV) model in rabbits. In the present study, a novel method is proposed to compare with two other methods. After 0.5 h of baseline two-lung ventilation (TLV), 40 rabbits were randomly divided into sham group (TLV for 3 h as a contrast) and three right-OLV groups (right OLV for 3 h with different methods): Deep intubation group, clamp group and blocker group (deeply intubate the self-made bronchial blocker into the left main bronchus, the novel method). These three methods were compared using a number of variables: Circulation by heart rate (HR), mean arterial pressure (MAP); oxygenation by arterial blood gas analysis; airway pressure; lung injury by histopathology; and time, blood loss, success rate of modeling. Following OLV, compared with the sham group, arterial partial pressure of oxygen and arterial hemoglobin oxygen saturation decreased, peak pressure increased and lung injury scores were higher in three OLV groups at 3 h of OLV. All these indexes showed no differences between the three OLV groups. During right‑OLV modeling, less time was spent in the blocker group (6±2 min), compared with the other two OLV groups (13±4 min in deep intubation group, P<0.05; 33±9 min in clamp group, P<0.001); more blood loss was observed in clamp group (11.7±2.8 ml), compared with the other two OLV groups (2.3±0.5 ml in deep intubation group, P<0.001; 2.1±0.6 ml in blocker group, P<0.001). The first-time and final success rate of modeling showed no differences among the three OLV groups. Deep intubation of the self‑made bronchial blocker into the left main bronchus is an easy, effective and reliable method to establish a right-OLV model in rabbits.

View Figures

View References

1	Della Rocca G and Coccia C: Ventilatory management of one-lung ventilation. Minerva Anestesiol. 77:534–536. 2011.PubMed/NCBI
2	Eguchi T, Hamanaka K, Kondo R, Saito G, Shiina T, Koizumi T and Yoshida K: Lung re-expansion following one-lung ventilation induces neutrophil cytoskeletal rearrangements in rats. Ann Thorac Cardiovasc Surg. 20:276–283. 2014. View Article : Google Scholar : PubMed/NCBI
3	Kozian A, Schilling T, Fredén F, Maripuu E, Röcken C, Strang C, Hachenberg T and Hedenstierna G: One-lung ventilation induces hyperperfusion and alveolar damage in the ventilated lung: An experimental study. Br J Anaesth. 100:549–559. 2008. View Article : Google Scholar : PubMed/NCBI
4	Kallet RH and Matthay MA: Hyperoxic acute lung injury. Respir Care. 58:123–141. 2013. View Article : Google Scholar : PubMed/NCBI
5	de Abreu MG and Pelosi P: How can we prevent postoperative pulmonary complications? Curr Opin Anaesthesiol. 26:105–106. 2013. View Article : Google Scholar : PubMed/NCBI
6	You Z, Feng D, Xu H, Cheng M, Li Z, Kan M and Yao S: Nuclear factor-kappa B mediates one-lung ventilation-induced acute lung injury in rabbits. J Invest Surg. 25:78–85. 2012. View Article : Google Scholar : PubMed/NCBI
7	Schilling T, Kretzschmar M, Hachenberg T, Hedenstier-Na G and Kozian A: The immune response to one-lung-ventilation is not affected by repeated alveolar recruitment manoeuvres in pigs. Minerva Anestesiol. 79:590–603. 2013.PubMed/NCBI
8	Adami C, Axiak S, Rytz U and Spadavecchia C: Alternating one lung ventilation using a double lumen endobronchial tube and providing CPAP to the non-ventilated lung in a dog. Vet Anaesth Analg. 38:70–76. 2011. View Article : Google Scholar : PubMed/NCBI
9	Siegl S and Uhlig S: Using the one-lung method to link p38 to pro-inflammatory gene expression during overventilation in C57BL/6 and BALB/c mice. PLoS One. 7:e414642012. View Article : Google Scholar : PubMed/NCBI
10	Siempos II, Maniatis NA, Kopterides P, Magkou C, Glynos C, Roussos C and Armaganidis A: Pretreatment with atorvastatin attenuates lung injury caused by high-stretch mechanical ventilation in an isolated rabbit lung model. Crit Care Med. 38:1321–1328. 2010. View Article : Google Scholar : PubMed/NCBI
11	Liu R, Yang Y, Li Y, Li J, Ma Q, Zhao Y and Wang D: Effects of sevoflurane on pulmonary cytosolic phospholipase A₂ and clara cell secretory protein expressions inrabbits with one-lung ventilation-induced lung injury. Nan Fang Yi Ke Da Xue Xue Bao. 33:469–473. 2013.PubMed/NCBI
12	Katz Y, Zisman E, Isserles SA and Rozenberg B: Left, but not right, one-lung ventilation causes hypoxemia during endoscopic transthoracic sympathectomy. J Cardiothorac Vasc Anesth. 10:207–209. 1996. View Article : Google Scholar : PubMed/NCBI
13	Schreiber T, Niemann C, Schmidt B and Karzai W: A novel model of selective lung ventilation to investigate the long-term effects of ventilation-induced lung injury. Shock. 26:50–54. 2006. View Article : Google Scholar : PubMed/NCBI
14	Nakamura M, Fujishima S, Sawafuji M, Ishizaka A, Oguma T, Soejima K, Matsubara H, Tasaka S, Kikuchi K, Kobayashi K, et al: Importance of interleukin-8 in the development of reexpansion lung injury in rabbits. Am J Respir Crit Care Med. 161:1030–1036. 2000. View Article : Google Scholar : PubMed/NCBI
15	Groh J, Kuhnle GE, Sckell A, Ney L and Goetz AE: Isoflurane inhibits hypoxic pulmonary vasoconstriction. An in vivo fluorescence microscopic study in rabbits. Anesthesiology. 81:1436–1444. 1994. View Article : Google Scholar : PubMed/NCBI
16	Liu D, Zeng BX and Shang Y: Decreased expression of peroxisome proliferator-activated receptor gamma in endotoxin-induced acute lung injury. Physiol Res. 55:291–299. 2006.PubMed/NCBI
17	Mayhew PD, Culp WT, Pascoe PJ, Kass PH and Johnson LR: Evaluation of blind thoracoscopic-assisted placement of three double-lumen endobronchial tube designs for one-lung ventilation in dogs. Vet Surg. 41:664–670. 2012. View Article : Google Scholar : PubMed/NCBI
18	Schwarzkopf K, Klein U, Schreiber T, Preussler NP, Bloos F, Helfritsch H, Sauer F and Karzai W: Oxygenation during one-lung ventilation: The effects of inhaled nitric oxide and increasing levels of inspired fraction of oxygen. Anesth Analg. 92:842–847. 2001. View Article : Google Scholar : PubMed/NCBI
19	Slinger P, Suissa S and Triolet W: Predicting arterial oxygenation during one-lung anaesthesia. Can J Anaesth. 39:1030–1035. 1992. View Article : Google Scholar : PubMed/NCBI
20	Kozian A, Schilling T, Fredén F, Maripuu E, Röcken C, Strang C, Hachenberg T and Hedenstierna G: One-lung ventilation induces hyperperfusion and alveolar damage in the ventilated lung: An experimental study. Br J Anaesth. 100:549–559. 2008. View Article : Google Scholar : PubMed/NCBI
21	Bhatia R, Shaffer TH, Hossain J, Fisher AO, Horner LM, Rodriguez ME, Penfil S and Theroux MC: Surfactant administration prior to one lung ventilation: Physiological and inflammatory correlates in a piglet model. Pediatr Pulmonol. 46:1069–1078. 2011. View Article : Google Scholar : PubMed/NCBI
22	Watanabe S, Noguchi E, Yamada S, Hamada N and Kano T: Sequential changes of arterial oxygen tension in the supine position during one-lung ventilation. Anesth Analg. 90:28–34. 2000. View Article : Google Scholar : PubMed/NCBI
23	Yin K, Gribbin E, Emanuel S, Orndorff R, Walker J, Weese J and Fallahnejad M: Histochemical alterations in one lung ventilation. J Surg Res. 137:16–20. 2007. View Article : Google Scholar : PubMed/NCBI
24	Della Rocca G and Coccia C: Acute lung injury in thoracic surgery. Curr Opin Anaesthesiol. 26:40–46. 2013. View Article : Google Scholar : PubMed/NCBI