Open Access

Effect of Lycium bararum polysaccharides on methylmercury-induced abnormal differentiation of hippocampal stem cells

  • Authors:
    • Jian‑Ying Tian
    • Wei‑Wei Chen
    • Jing Cui
    • Hao Wang
    • Ci Chao
    • Zhi‑Yan Lu
    • Yong‑Yi Bi
  • View Affiliations

  • Published online on: June 2, 2016     https://doi.org/10.3892/etm.2016.3415
  • Pages: 683-689
  • Copyright: © Tian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to observe the effects of a general extract of Lycium bararum polysaccharides (LBPs) on methylmercury (MeHg)-induced damage in hippocampus neural stem cells (hNSCs). The hippocampal tissues of embryonic day 16 Sprague-Dawley rats were extracted for the isolation, purification and cloning of hNSCs. Following passage and proliferation for 10 days, the cells were allocated at random into the following groups: Control, LBPs, MeHg and MeHg + LBPs. MTT and microtubule‑associated protein 2 (MAP‑2)/glial fibrillary acidic protein/Hoechst immunofluorescence tests were performed to detect the differentiation and growth of hNSCs in the various groups. The differentiation rate of MeHg-treated hNSCs and the perimeter of MAP‑2‑positive neurons were 3.632±0.63% and 62.36±5.58 µm, respectively, significantly lower compared with the control group values of 6.500±0.81% and 166±8.16 µm (P<0.05). Furthermore, the differentiation rate and the perimeter of MAP‑2‑positive neurons in LBPs groups cells was 7.75±0.59% and 253.3±11.21 µm, respectively, significantly higher compared with the control group (P<0.05). The same parameters in the MeHg + LBPs group were 5.92±0.98% and 111.9±6.07 µm, respectively, significantly higher than the MeHg group (P<0.05). The astrocyte differentiation rates in the MeHg and MeHg + LBPs group were 41.19±2.14 and 34.58±1.70, respectively (P<0.05). These results suggest that LBPs may promote the generation and development of new neurons and inhibit the MeHg‑induced abnormal differentiation of astrocytes. Thus, LBPs may be considered to be a potential new treatment for MeHg-induced neurotoxicity in hNSCs.
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August-2016
Volume 12 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Tian JY, Chen WW, Cui J, Wang H, Chao C, Lu ZY and Bi YY: Effect of Lycium bararum polysaccharides on methylmercury-induced abnormal differentiation of hippocampal stem cells. Exp Ther Med 12: 683-689, 2016
APA
Tian, J., Chen, W., Cui, J., Wang, H., Chao, C., Lu, Z., & Bi, Y. (2016). Effect of Lycium bararum polysaccharides on methylmercury-induced abnormal differentiation of hippocampal stem cells. Experimental and Therapeutic Medicine, 12, 683-689. https://doi.org/10.3892/etm.2016.3415
MLA
Tian, J., Chen, W., Cui, J., Wang, H., Chao, C., Lu, Z., Bi, Y."Effect of Lycium bararum polysaccharides on methylmercury-induced abnormal differentiation of hippocampal stem cells". Experimental and Therapeutic Medicine 12.2 (2016): 683-689.
Chicago
Tian, J., Chen, W., Cui, J., Wang, H., Chao, C., Lu, Z., Bi, Y."Effect of Lycium bararum polysaccharides on methylmercury-induced abnormal differentiation of hippocampal stem cells". Experimental and Therapeutic Medicine 12, no. 2 (2016): 683-689. https://doi.org/10.3892/etm.2016.3415