Role of rat autologous skin fibroblasts and mechanism underlying the repair of depressed scars

Zhao,Juan; Liu,Yan‑Chun; Shi,Yan‑Hua; Xie,Ya‑Qin; Cui,Hai‑Peng; Li,Ying; Li,Xiang‑Jun; Ren,Li‑Qun

doi:10.3892/etm.2016.3442

Role of rat autologous skin fibroblasts and mechanism underlying the repair of depressed scars

Authors:
- Juan Zhao
- Yan‑Chun Liu
- Yan‑Hua Shi
- Ya‑Qin Xie
- Hai‑Peng Cui
- Ying Li
- Xiang‑Jun Li
- Li‑Qun Ren
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Affiliations: Department of Pathophysiology, Chengde Medical College, Chengde, Hebei 067000, P.R. China, Department of Experimental Pharmacology and Toxicology, Medical School of Jilin University, Changchun, Jilin 130021, P.R. China
Published online on: June 8, 2016 https://doi.org/10.3892/etm.2016.3442
Pages: 945-950
Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to provide reliable experimental evidence for the application of autologous skin fibroblasts (asFbs) in the repair of depressed scars. In the experiments, depressed trauma was induced in male Wistar rats, and fibroblasts were separated from the removed skin tissues to culture in medium. In vitro cultured asFbs were injected into the depressed scar sites of rats, and the repair function of asFbs in the depressed scars was then examined at the cellular and whole‑animal levels. The expression levels of type I and type III collagen in the dermal layer of the skin injected with asFb cells were significantly higher, as compared with those of the control, and type I collagen expression was significantly higher compared with Type III. Re‑injection of asFbs into the dermal layer of depressed scars can markedly improve their repair. These results may prove useful for skin repair in clinical settings.

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1	Zeng W, Wei ZR, Liu D, Chai M and Zhao YM: Preliminary clinical observations on autologous cultured skin fibroblasts transplantation to treat the facial soft tissue deficiencies. Zhonghua Zheng Xing Wai Ke Za Zhi. 29:29–33. 2013.(In Chinese). PubMed/NCBI
2	Ren HT, Hu H, Li Y, Jiang HF, Hu XL and Han CM: Endostatin inhibits hypertrophic scarring in a rabbit ear model. J Zhejiang Univ Sci B. 14:224–230. 2013. View Article : Google Scholar : PubMed/NCBI
3	Jung DH, Medikeri GS, Chang GU and Hyun SM: Surgical techniques for the correction of postrhinoplasty depressed scars on the nasal tip. JAMA Facial Plast Surg. 17:405–412. 2015. View Article : Google Scholar : PubMed/NCBI
4	Seok J, Choi SY, Park KY, Jang JH, Bae JH, Kim BJ, Kim MN and Hong CK: Depressed scar after filler injection successfully treated with pneumatic needleless injector and radiofrequency device. Dermatol Ther. 29:45–47. 2016. View Article : Google Scholar : PubMed/NCBI
5	Skigen AL, Bedrock RD and Stopperich PS: Correction of the depressed, retracted, post-tracheostomy scar. Plast Reconstr Surg. 103:1703–1705. 1999. View Article : Google Scholar : PubMed/NCBI
6	Inchingolo F, Tatullo M, Abenavoli FM, Marrelli M, Inchingolo AD, Corelli R, Inchingolo AM and Dipalma G: Surgical treatment of depressed scar: A simple technique. Int J Med Sci. 8:377–379. 2011. View Article : Google Scholar : PubMed/NCBI
7	Khan F, Richards K and Rashid RM: Hyaluronic acid filler for a depressed scar. Dermatol Online J. 18:152012.PubMed/NCBI
8	Kubo K and Kuroyanagi Y: A study of cytokines released from fibroblasts in cultured dermal substitute. Artif Organs. 29:845–849. 2005. View Article : Google Scholar : PubMed/NCBI
9	Burd A and Chiu T: Allogeneic skin in the treatment of burns. Clin Dermatol. 23:376–387. 2005. View Article : Google Scholar : PubMed/NCBI
10	Chang H, Chi JT, Dudoit S, Bondre C, van de Rijn M, Botstein D and Brown PO: Diversity, topographic differentiation, and positional memory in human fibroblasts. Proc Natl Acad Sci USA. 99:12877–12882. 2002. View Article : Google Scholar : PubMed/NCBI
11	Clark DP, Hanke CW and Swanson NA: Dermal implants: Safety of products injected for soft tissue augmentation. J Am Aced Dermatol. 21:992–998. 1989. View Article : Google Scholar
12	Viyoch J, Buranajaree S, Grandmottet F, Robin S, Binda D, Viennet C, Waranuch N and Humbert P: Evaluation of the effect of Thai breadfruit's heartwood extract on the biological functions of fibroblasts from wrinkles. J Cosmet Sci. 61:311–324. 2010.PubMed/NCBI
13	Kotaska K, Petrak B, Kukacka J, Kraus J and Prusa R: Anti-vimentin antibodies and neuron-specific enolase in children with neurofibromatosis type-1. Neuro Endocrinol Lett. 28:761–764. 2007.PubMed/NCBI
14	Chen X, Xu R, Jiang YN, Zhu WN and Wang YH: Simultaneous separation of primary cardiomyocytes and cardiac fibroblasts from neonatal rats with density gradient centrifugation. Sheng Li Xue Bao. 67:423–430. 2015.(In Chinese). PubMed/NCBI
15	Avelar-Freitas BA, Almeida VG, Pinto MC, Mourão FA, Massensini AR, Martins-Filho OA, Rocha-Vieira E and Brito-Melo GE: Trypan blue exclusion assay by flow cytometry. Braz J Med Biol Res. 47:307–315. 2014. View Article : Google Scholar : PubMed/NCBI
16	Suto A, Kubota T, Shimoyama Y, Ishibiki K and Abe O: MTT assay with reference to the clinical effect of chemotherapy. J Surg Oncol. 42:28–32. 1989. View Article : Google Scholar : PubMed/NCBI
17	Werely WA: Automated hematoxylin and eosin staining for large volumes of tissue. Am J Med Technol. 42:285–287. 1976.PubMed/NCBI
18	Cooper JS and Lee BT: Treatment of facial scarring: lasers, filler, and nonoperative techniques. Facial Plast Surg. 25:311–315. 2009. View Article : Google Scholar : PubMed/NCBI
19	Han HH, Kim SY, Lee YJ, Moon SH and Oh DY: Donor-site closure using absorbable dermal staple for deep inferior epigastric artery perforator flaps: Its efficacy and cosmetic outcomes. Springerplus. 23:3632016. View Article : Google Scholar
20	Byun SH, Ahn KM, Kim SM and Lee JH: Functional and cosmetic outcome after closure of radial forearm free flap donor defect with porcine collagen membrane. J Craniomaxillofac Surg. 44:527–532. 2016. View Article : Google Scholar : PubMed/NCBI
21	Tanaydin V, Conings J, Malyar M, van der Hulst R and van der Lei B: The role of topical vitamin E in scar management: A systematic review. Aesthet Surg J. Mar 14–2016.(Epub ahead of print) pii: sjw046. View Article : Google Scholar : PubMed/NCBI
22	Munavalli GS, Smith S, Maslowski JM and Weiss RA: Successful treatment of depressed, distensible acne scars using autologous fibroblasts: A multi-site, prospective, double blind, placebo-controlled clinical trial. Dermatol Surg. 39:1226–1236. 2013. View Article : Google Scholar : PubMed/NCBI
23	McAnulty RJ: Fibroblasts and myofibroblasts: Their source, function and role in disease. Int J Biochem Cell Biol. 39:666–671. 2007. View Article : Google Scholar : PubMed/NCBI
24	Pan S, Chen W, Liu X, Xiao J, Wang Y, Liu J, Du Y, Wang Y and Zhang Y: Application of a novel population of multipotent stem cells derived from skin fibroblasts as donor cells in bovine SCNT. PLoS One. 10:e01144232015. View Article : Google Scholar : PubMed/NCBI
25	Doucet YS and Owens DM: Isolation and functional assessment of cutaneous stem cells. Methods Mol Biol. 1235:147–164. 2015. View Article : Google Scholar : PubMed/NCBI
26	Tondato F, Zeng H, Goodchild T, Ng FS, Chronos N and Peters NS: Autologous dermal fibroblast injections slow atrioventricular conduction and ventricular rate in atrial fibrillation in swine. Circ Arrhythm Electrophysiol. 8:439–446. 2015. View Article : Google Scholar : PubMed/NCBI
27	Walmsley GG, Maan ZN, Hu MS, Atashroo DA, Whittam AJ, Duscher D, TEvlin R, Marecic O, Lorenz HP, Gurtner GC and Longaker MT: Murine dermal fibroblast isolation by FACS. J Vis Exp. 107:e534302016.