Separation of cordycepin from Cordyceps militaris fermentation supernatant using preparative HPLC and evaluation of its antibacterial activity as an NAD+-dependent DNA ligase inhibitor

  • Authors:
    • Xiaofeng Zhou
    • Guoqiang Cai
    • Yi He
    • Guotong Tong
  • View Affiliations

  • Published online on: July 20, 2016     https://doi.org/10.3892/etm.2016.3536
  • Pages: 1812-1816
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Abstract

Cordycepin exhibits various bio-activities, including anticancer, antibacterial, antiviral and immune regulation activities, and is a significant focus of research. However, the preparation of high-purity cordycepin remains challenging. Also, the molecular target with which cordycepin interacts to cause an antibacterial effect remains unknown. In the present study, cordycepin was prepared by preparative high‑performance liquid chromatography (prep-HPLC) and the purity obtained was 99.6%, indicating that this technique may be useful for the large‑scale isolation of cordycepin in the future. The results of computational molecular docking analysis indicated that the interaction energy between cordycepin and NAD+‑dependent DNA ligase (LigA) was lower than that between cordycepin and other common antibacterial targets. The highly pure cordycepin obtained by prep‑HPLC demonstrated inhibitory activity against LigA from various bacteria in vitro. In conclusion, cordycepin may be useful as a broad‑spectrum antibiotic targeting LigA in various bacteria.

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September 2016
Volume 12 Issue 3

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APA
Zhou, X., Cai, G., He, Y., & Tong, G. (2016). Separation of cordycepin from Cordyceps militaris fermentation supernatant using preparative HPLC and evaluation of its antibacterial activity as an NAD+-dependent DNA ligase inhibitor. Experimental and Therapeutic Medicine, 12, 1812-1816. https://doi.org/10.3892/etm.2016.3536
MLA
Zhou, X., Cai, G., He, Y., Tong, G."Separation of cordycepin from Cordyceps militaris fermentation supernatant using preparative HPLC and evaluation of its antibacterial activity as an NAD+-dependent DNA ligase inhibitor". Experimental and Therapeutic Medicine 12.3 (2016): 1812-1816.
Chicago
Zhou, X., Cai, G., He, Y., Tong, G."Separation of cordycepin from Cordyceps militaris fermentation supernatant using preparative HPLC and evaluation of its antibacterial activity as an NAD+-dependent DNA ligase inhibitor". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1812-1816. https://doi.org/10.3892/etm.2016.3536