Targeting CRMP‑4 by lentivirus‑mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth

Chen,Si‑Le; Cai,Shi‑Rong; Zhang,Xin‑Hua; Li,Wen‑Feng; Zhai,Er‑Tao; Peng,Jian‑Jun; Wu,Hui; Chen,Chuang‑Qi; Ma,Jin‑Ping; Wang,Zhao; He,Yu‑Long

doi:10.3892/etm.2016.3588

Targeting CRMP‑4 by lentivirus‑mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth

Authors:
- Si‑Le Chen
- Shi‑Rong Cai
- Xin‑Hua Zhang
- Wen‑Feng Li
- Er‑Tao Zhai
- Jian‑Jun Peng
- Hui Wu
- Chuang‑Qi Chen
- Jin‑Ping Ma
- Zhao Wang
- Yu‑Long He
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Affiliations: Department of Gastrointestinal and Pancreatic Surgery, The First Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China
Published online on: August 10, 2016 https://doi.org/10.3892/etm.2016.3588
Pages: 2003-2008
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the expression level of collapsin response mediator protein 4 (CRMP‑4) in human colorectal cancer (CRC) tissue and to evauluate its impact on SW480 cell proliferation, in addition to tumor growth in a mouse xenograft model. Clinical CRC tissue samples were collected to detect the CRMP‑4 protein expression levels using western blot and immunohistochemistry analyses. A specific small interfering RNA sequence targeting the CRMP‑4 gene (DPYSL3) was constructed and transfected into an SW480 cell line using a lentivirus vector to obtain a stable cell line with low expression of CRMP‑4. The effectiveness of the interference was evaluated using western blot and reverse transcription‑quantitative polymerase chain reaction, and the cell proliferation was determined using MTT and BrdU colorimetric methods. Tumor growth was assessed by subcutaneously inoculating the constructed cells into BALB/c nude mice. The protein expression levels of CRMP‑4 were markedly increased in colon tumor tissue of the human samples. The proliferation of SW480 cells and the tumor growth rate in nude mice of the si‑CPMR‑4 group were evidently depressed compared with the si‑scramble group. Thus, the present results suggest that CRMP‑4 may be involved in the pathogenesis of CRC.

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