Glycitin regulates osteoblasts through TGF-β or AKT signaling pathways in bone marrow stem cells

  • Authors:
    • Liyan Zhang
    • Jiying Chen
    • Wei Chai
    • Min Ni
    • Xin Sun
    • Dan Tian
  • View Affiliations

  • Published online on: September 13, 2016     https://doi.org/10.3892/etm.2016.3696
  • Pages: 3063-3067
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Abstract

The aim of the present study was to examine the effect of glycitin on the regulation of osteoblasts from bone marrow stem cells (BMSCs) through transforming growth factor (TGF)‑β or protein kinase B (AKT) signaling pathways. BMSCs were extracted from New Zealand white rabbits and used to analyze the effect of glycitin on BMSCs. BMSCs were cleared using xylene and observed via light microscopy. BMSCs were subsequently induced with glycitin (0.01, 0.5, 1, 5 and 10 µM) for 7 days, and stained with Oil Red O. The mechanism of action of glycitin on BMSCs was investigated, in which contact with collagen type I (Col I), alkaline phosphatase (ALP), TGF‑β and AKT was studied. Firstly, BMSCs appeared homogeneously mazarine blue, and which showed that BMSCs were successful extracted. Administration of glycitin increased cell proliferation and promoted osteoblast formation from BMSCs. Furthermore, glycitin activated the gene expression of Col I and ALP in BMSCs. Notably, glycitin suppressed protein expression of TGF‑β and AKT in BMSCs. These results indicated that glycitin may regulate osteoblasts through TGF‑β or AKT signaling pathways in BMSCs.
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November-2016
Volume 12 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhang L, Chen J, Chai W, Ni M, Sun X and Tian D: Glycitin regulates osteoblasts through TGF-β or AKT signaling pathways in bone marrow stem cells. Exp Ther Med 12: 3063-3067, 2016
APA
Zhang, L., Chen, J., Chai, W., Ni, M., Sun, X., & Tian, D. (2016). Glycitin regulates osteoblasts through TGF-β or AKT signaling pathways in bone marrow stem cells. Experimental and Therapeutic Medicine, 12, 3063-3067. https://doi.org/10.3892/etm.2016.3696
MLA
Zhang, L., Chen, J., Chai, W., Ni, M., Sun, X., Tian, D."Glycitin regulates osteoblasts through TGF-β or AKT signaling pathways in bone marrow stem cells". Experimental and Therapeutic Medicine 12.5 (2016): 3063-3067.
Chicago
Zhang, L., Chen, J., Chai, W., Ni, M., Sun, X., Tian, D."Glycitin regulates osteoblasts through TGF-β or AKT signaling pathways in bone marrow stem cells". Experimental and Therapeutic Medicine 12, no. 5 (2016): 3063-3067. https://doi.org/10.3892/etm.2016.3696